Behavioral plasticity is widely thought to require both protein synthesis and protein turnover. Although several studies indicate that protein homeostasis is tightly regulated and that new protein synthesis can have rapid effects on synaptic plasticity, aside from a few well-studied examples, the identification and function of newly- synthesized proteins is still limited. We propose multidisciplinary experiments using state of the art proteomics to identify newly-synthesized proteins induced in response to conditioning stimuli, to probe the time course for protein synthesis in conditioning- induced behavioral plasticity of a visuomotor task, and to test whether the rapid synthesis of key proteins plays a role in plasticity. Results from the proposed experiments will illuminate novel mechanisms of brain plasticity and may have bearing on treatments for neurodevelopmental disorders, such as autism spectrum disorders, or neurorepair following injury.

Public Health Relevance

Protein synthesis is thought to be required for behavioral plasticity. We propose experiments to identify proteins that are newly synthesized during behavioral training and to determine their function in the brain.

Agency
National Institute of Health (NIH)
Type
Research Project (R01)
Project #
2R01EY011261-19
Application #
8701656
Study Section
(SPC)
Program Officer
Greenwell, Thomas
Project Start
Project End
Budget Start
Budget End
Support Year
19
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Scripps Research Institute
Department
Type
DUNS #
City
La Jolla
State
CA
Country
United States
Zip Code
92037
Shen, Wanhua; Liu, Han-Hsuan; Schiapparelli, Lucio et al. (2014) Acute synthesis of CPEB is required for plasticity of visual avoidance behavior in Xenopus. Cell Rep 6:737-47
Hiramoto, Masaki; Cline, Hollis T (2014) Optic flow instructs retinotopic map formation through a spatial to temporal to spatial transformation of visual information. Proc Natl Acad Sci U S A 111:E5105-13
Bestman, Jennifer E; Cline, Hollis T (2014) Morpholino studies in Xenopus brain development. Methods Mol Biol 1082:155-71
McKeown, Caroline R; Sharma, Pranav; Sharipov, Heidi E et al. (2013) Neurogenesis is required for behavioral recovery after injury in the visual system of Xenopus laevis. J Comp Neurol 521:2262-78
Lee, Ping-Chang; He, Hai-Yan; Lin, Chih-Yang et al. (2013) Computer aided alignment and quantitative 4D structural plasticity analysis of neurons. Neuroinformatics 11:249-57
Sharma, Pranav; Schiapparelli, Lucio; Cline, Hollis T (2013) Exosomes function in cell-cell communication during brain circuit development. Curr Opin Neurobiol 23:997-1004
Bestman, Jennifer E; Lee-Osbourne, Jane; Cline, Hollis T (2012) In vivo time-lapse imaging of cell proliferation and differentiation in the optic tectum of Xenopus laevis tadpoles. J Comp Neurol 520:401-33
Miraucourt, Lois S; Silva, Jorge Santos da; Burgos, Kasandra et al. (2012) GABA expression and regulation by sensory experience in the developing visual system. PLoS One 7:e29086
Shen, Wanhua; McKeown, Caroline R; Demas, James A et al. (2011) Inhibition to excitation ratio regulates visual system responses and behavior in vivo. J Neurophysiol 106:2285-302
Li, Jianli; Erisir, Alev; Cline, Hollis (2011) In vivo time-lapse imaging and serial section electron microscopy reveal developmental synaptic rearrangements. Neuron 69:273-86

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