Vision depends on the accurate processing of the image by the neural retina. The development of the retina involves a series of events that result in the proper wiring of its circuitry. The first step in building retinal circuits is the generation of each major class of retinal neuron and subsequently, establishing the appropriate connectivity between the cell components. Our understanding of the mechanisms that regulate these major steps in development is not yet complete. In particular, it has not been possible to determine the cellular processes involved in dynamically shaping the number, location and wiring patterns of retinal neurons throughout their entire time-course of development in vivo. This knowledge is important as the consequences of disrupting cellular function during development is more readily identified when we have a real-time view of the developmental events as they occur in the live animal. It is now possible to follow the development of retinal neurons from the time of their genesis to when they form circuits in the rapidly developing and largely transparent zebrafish embryo. In this proposal, we will focus on a major cell component of the outer retina, the horizontal cells, and determine:
(Aim 1) how an unconventional mode of neurogenesis produces the right number of horizontal cells, and how migration of the daughter cells lead to the formation of their mosaic-like network, and (Aim 2) determine what cell-cell interactions, involving neurons and Muller glial cells, are important for establishing horizontal cell connectivity with cone photoreceptors. Our approach is to use time-lapse multiphoton imaging of fluorescently labeled retinal neurons to track their behavior over hours to days, and a targeted cell ablation approach using the titanium:sapphire infrared laser to unravel cell-cell interactions. Together, the proposed in vivo studies will increase our understanding of the cellular behaviors and interactions necessary for establishing normal retinal networks and provide the foundation for future investigations on retinal disease and injury.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY014358-09
Application #
7762209
Study Section
Biology and Diseases of the Posterior Eye Study Section (BDPE)
Program Officer
Greenwell, Thomas
Project Start
2003-02-01
Project End
2012-01-31
Budget Start
2010-02-01
Budget End
2011-01-31
Support Year
9
Fiscal Year
2010
Total Cost
$347,490
Indirect Cost
Name
University of Washington
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
Engerer, Peter; Suzuki, Sachihiro C; Yoshimatsu, Takeshi et al. (2017) Uncoupling of neurogenesis and differentiation during retinal development. EMBO J 36:1134-1146
Zhang, Chi; Kolodkin, Alex L; Wong, Rachel O et al. (2017) Establishing Wiring Specificity in Visual System Circuits: From the Retina to the Brain. Annu Rev Neurosci 40:395-424
Yoshimatsu, Takeshi; D'Orazi, Florence D; Gamlin, Clare R et al. (2016) Presynaptic partner selection during retinal circuit reassembly varies with timing of neuronal regeneration in vivo. Nat Commun 7:10590
D'Orazi, Florence D; Zhao, Xiao-Feng; Wong, Rachel O et al. (2016) Mismatch of Synaptic Patterns between Neurons Produced in Regeneration and during Development of the Vertebrate Retina. Curr Biol 26:2268-79
MacDonald, Ryan B; Randlett, Owen; Oswald, Julia et al. (2015) Müller glia provide essential tensile strength to the developing retina. J Cell Biol 210:1075-83
Okawa, Haruhisa; Hoon, Mrinalini; Yoshimatsu, Takeshi et al. (2014) Illuminating the multifaceted roles of neurotransmission in shaping neuronal circuitry. Neuron 83:1303-1318
Hoon, Mrinalini; Okawa, Haruhisa; Della Santina, Luca et al. (2014) Functional architecture of the retina: development and disease. Prog Retin Eye Res 42:44-84
Engerer, Peter; Yoshimatsu, Takeshi; Suzuki, Sachihiro C et al. (2014) CentrinFish permit the visualization of centrosome dynamics in a cellular context in vivo. Zebrafish 11:586-7
Yoshimatsu, Takeshi; Williams, Philip R; D'Orazi, Florence D et al. (2014) Transmission from the dominant input shapes the stereotypic ratio of photoreceptor inputs onto horizontal cells. Nat Commun 5:3699
Almeida, Alexandra D; Boije, Henrik; Chow, Renee W et al. (2014) Spectrum of Fates: a new approach to the study of the developing zebrafish retina. Development 141:1971-80

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