Abstract

We will continue to study the molecular mechanisms involved in the erasure process of Dictyostelium discoideum, a dedifferentiation involving the programmed loss of chemotactic functions. In particular, we will examine the target for cAMP inhibition of erasure and the membrane changes associated with the erasure event. Changes in gene expression will be examined both in vivo and in vitro, employing a rabbit reticulocyte system for protein synthesis. We will also continue to develop and apply methods for examining the complexity and relationships of developmental timers. The methods will be applied to Dictyostelium morphogenesis, yeast budding, and water mold sporulation. Analysis of timer mutants will continue.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM025832-07
Application #
3273337
Study Section
Molecular Cytology Study Section (CTY)
Project Start
1979-09-01
Project End
1986-08-31
Budget Start
1985-09-01
Budget End
1986-08-31
Support Year
7
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Iowa
Department
Type
Schools of Arts and Sciences
DUNS #
041294109
City
Iowa City
State
IA
Country
United States
Zip Code
52242

  Publications

Chandrasekhar, A; Ennis, H L; Soll, D R (1992) Biological and molecular correlates between induced dedifferentiation and spore germination in Dictyostelium. Development 116:417-25
Fukui, Y; Murray, J; Riddelle, K S et al. (1991) Cell behavior and actomyosin organization in Dictyostelium during substrate exploration. Cell Struct Funct 16:289-301
Chandrasekhar, A; Rotman, M; Kraft, B et al. (1990) Developmental mechanisms regulating the rapid decrease in a cohesion glycoprotein mRNA in Dictyostelium function primarily at the level of mRNA degradation. Dev Biol 141:262-9
Soll, D R; Wessels, D; Murray, J et al. (1990) Intracellular vesicle movement, cAMP and myosin II in Dictyostelium. Dev Genet 11:341-53
Wessels, D; Schroeder, N A; Voss, E et al. (1989) cAMP-mediated inhibition of intracellular particle movement and actin reorganization in Dictyostelium. J Cell Biol 109:2841-51
Kraft, B; Chandrasekhar, A; Rotman, M et al. (1989) Dictyostelium erasure mutant HI4 abnormally retains development-specific mRNAs during dedifferentiation. Dev Biol 136:363-71
Wessels, D; Soll, D R; Knecht, D et al. (1988) Cell motility and chemotaxis in Dictyostelium amebae lacking myosin heavy chain. Dev Biol 128:164-77
Soll, D R; Voss, E; Varnum-Finney, B et al. (1988) ""Dynamic Morphology System"": a method for quantitating changes in shape, pseudopod formation, and motion in normal and mutant amoebae of Dictyostelium discoideum. J Cell Biochem 37:177-92
Kraft, B; Steinbrech, D; Yang, M et al. (1988) High-frequency switching in Dictyostelium. Dev Biol 130:198-208
Varnum-Finney, B; Schroeder, N A; Soll, D R (1988) Adaptation in the motility response to cAMP in Dictyostelium discoideum. Cell Motil Cytoskeleton 9:9-16

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