The C. elegans model system is ideal for studying the genetic and molecular mechanisms of the control of cell division and differentiation in vivo. We focus on the epidermal """"""""seam cells"""""""" that undergo a stereotyped pattern of divisions throughout larval development, followed by terminal differentiation at adulthood. Seam cells exhibit stem-cell- like behavior, including self-renewal through either asymmetric or symmetric cell division. The execution of symmetric and asymmetric cell divisions by seam cells is regulated by developmental timing mechanisms that have been extensively characterized in our lab. In the first Aim of the project, we will explore how developmental timing regulators interface with pathways affecting cell division polarity to control when seam cells switch from asymmetric to symmetric cell division.
The second Aim will extend these studies to identify new genes that affect the regulation of symmetric vs. asymmetic cell divisions in seam cells.
The third Aim will utilize C. elegans as a model to study the mechanisms governing stem cell quiescence. Mammalian adult stem cells commonly transition between proliferative and quiescent states, depending on extracellular signals. During quiescence, a stem cell's vitality and developmental potential is maintained for extended periods of time. We propose to learn about mechanisms that controlling stem cell maintenance during quiescence in dauer larva developmental arrest. The dauer larva is an optional developmentally-arrested second stage larva that is induced in response to environmental conditions unfavorable for growth. In dauer larvae, the seam cells enter quiescence, and remarkably, this quiescence profoundly affects how seam cells respond to certain developmental regulatory genes, including the evolutionarily conserved lin-4 and let-7 microRNA genes. Studying how developmental quiescence interacts with developmental timing regulators in C. elegans cell lineages should reveal fundamental principles of stem cell behavior relevant to human biology, including development, cancer, tissue homeostasis and wound healing.
C. elegans is an excellent system for the genetic analysis of developmental processes in animals, including the control of cell division, cell number, and cellular differentiation. Since many of the proteins and other regulatory molecules that control C. elegans development are also found in mammals, understanding their roles in C. elegans should reveal the mechanisms and principles underlying developmental processes common to all animals, including humans.
|Ambros, Victor; Ruvkun, Gary (2018) Recent Molecular Genetic Explorations of Caenorhabditis elegans MicroRNAs. Genetics 209:651-673|
|McJunkin, Katherine; Ambros, Victor (2017) A microRNA family exerts maternal control on sex determination in C. elegans. Genes Dev 31:422-437|
|Ren, Zhiji; Veksler-Lublinsky, Isana; Morrissey, David et al. (2016) Staufen Negatively Modulates MicroRNA Activity in Caenorhabditis elegans. G3 (Bethesda) 6:1227-37|
|Burke, Samantha L; Hammell, Molly; Ambros, Victor (2015) Robust Distal Tip Cell Pathfinding in the Face of Temperature Stress Is Ensured by Two Conserved microRNAS in Caenorhabditis elegans. Genetics 200:1201-18|
|Ren, Zhiji; Ambros, Victor R (2015) Caenorhabditis elegans microRNAs of the let-7 family act in innate immune response circuits and confer robust developmental timing against pathogen stress. Proc Natl Acad Sci U S A 112:E2366-75|
|Sterling, Catherine H; Veksler-Lublinsky, Isana; Ambros, Victor (2015) An efficient and sensitive method for preparing cDNA libraries from scarce biological samples. Nucleic Acids Res 43:e1|
|Zinovyeva, Anna Y; Veksler-Lublinsky, Isana; Vashisht, Ajay A et al. (2015) Caenorhabditis elegans ALG-1 antimorphic mutations uncover functions for Argonaute in microRNA guide strand selection and passenger strand disposal. Proc Natl Acad Sci U S A 112:E5271-80|
|Harandi, Omid F; Ambros, Victor R (2015) Control of stem cell self-renewal and differentiation by the heterochronic genes and the cellular asymmetry machinery in Caenorhabditis elegans. Proc Natl Acad Sci U S A 112:E287-96|
|Nelson, Charles; Ambros, Victor; Baehrecke, Eric H (2014) miR-14 regulates autophagy during developmental cell death by targeting ip3-kinase 2. Mol Cell 56:376-88|
|Zinovyeva, Anna Y; Bouasker, Samir; Simard, Martin J et al. (2014) Mutations in conserved residues of the C. elegans microRNA Argonaute ALG-1 identify separable functions in ALG-1 miRISC loading and target repression. PLoS Genet 10:e1004286|
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