The overall goal of this proposal is to further understand events in the Drosophila embryo that play a role in the modulation of screw (scw) activity and its spatial restriction to dorsal cells. Scw is a member of the TGF family whose RNA is uniformly localized, but its activity is not required in the ventral region. There are three models: (1) that scw and dorsally localized dpp form heterodimers that are necessary for dorsal structures; (2) that tolloid, a BMP-1 like metalloprotease that is dorsally localized, is necessary to process scw to its active form; and (3) that ventral short gastrulation prevents scw function. There are four aims.
Specific Aim #1 centers on structure-function relationships in the scw protein.
Specific Aim #2 addresses the possibility of scw involvement in later stages of development.
Specific Aim #3 focuses on the establishment of molecular assays to test models for modulation of scw activity.
Specific Aim #4 is to characterize scw biochemically.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM055442-03
Application #
2750136
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1996-08-01
Project End
2001-07-31
Budget Start
1998-08-01
Budget End
1999-07-31
Support Year
3
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of California Irvine
Department
Anatomy/Cell Biology
Type
Schools of Arts and Sciences
DUNS #
161202122
City
Irvine
State
CA
Country
United States
Zip Code
92697
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