R01-GM57587, which supports our mechanistic studies of SCF and APC/C ubiquitin ligases, was severely compromised by the hurricane Sandy. This application represents a request of a twelve months cost extension to allow us to recover the time lost and re-generate valuable lost reagents that were generated during the first two years after this grant was competitively renewed. These home-made reagents are absolutely required to perform the projects covered by R01-GM57587.

Public Health Relevance

Cells depend on the proper functioning of an ensemble of networked, molecular machines to control diverse processes, ranging from cell proliferation to cell death to differentiation. The ubiquitin system can rapidly degrade the modular regulatory components of these machines, contributing to the precise operation and synchronization of complex cellular processes. Given its critical role, the ubiquitin system is often deregulated in cancer cells. Thus, it is anticipated that the results of the proposed studies will have an impact on both basic science and cancer biology.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
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Hamlet, Michelle R
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New York University
Schools of Medicine
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Dankert, John F; Rona, Gergely; Clijsters, Linda et al. (2016) Cyclin F-Mediated Degradation of SLBP Limits H2A.X Accumulation and Apoptosis upon Genotoxic Stress in G2. Mol Cell 64:507-519
Young, Lauren M; Marzio, Antonio; Perez-Duran, Pablo et al. (2015) TIMELESS Forms a Complex with PARP1 Distinct from Its Complex with TIPIN and Plays a Role in the DNA Damage Response. Cell Rep 13:451-9
Duan, Shanshan; Pagano, Michele (2015) SPOP Mutations or ERG Rearrangements Result in Enhanced Levels of ERG to Promote Cell Invasion in Prostate Cancer. Mol Cell 59:883-4
Skaar, Jeffrey R; Ferris, Andrea L; Wu, Xiaolin et al. (2015) The Integrator complex controls the termination of transcription at diverse classes of gene targets. Cell Res 25:288-305
Pagan, Julia K; Marzio, Antonio; Jones, Mathew J K et al. (2015) Degradation of Cep68 and PCNT cleavage mediate Cep215 removal from the PCM to allow centriole separation, disengagement and licensing. Nat Cell Biol 17:31-43
Horn, Moritz; Geisen, Christoph; Cermak, Lukas et al. (2014) DRE-1/FBXO11-dependent degradation of BLMP-1/BLIMP-1 governs C. elegans developmental timing and maturation. Dev Cell 28:697-710
Choi, Byeong Hyeok; Pagano, Michele; Huang, Chaunshu et al. (2014) Cdh1, a substrate-recruiting component of anaphase-promoting complex/cyclosome (APC/C) ubiquitin E3 ligase, specifically interacts with phosphatase and tensin homolog (PTEN) and promotes its removal from chromatin. J Biol Chem 289:17951-9
Skaar, Jeffrey R; Pagan, Julia K; Pagano, Michele (2014) SCF ubiquitin ligase-targeted therapies. Nat Rev Drug Discov 13:889-903
Bassermann, Florian; Eichner, Ruth; Pagano, Michele (2014) The ubiquitin proteasome system - implications for cell cycle control and the targeted treatment of cancer. Biochim Biophys Acta 1843:150-62
Kanarek, Naama; Grivennikov, Sergei I; Leshets, Michael et al. (2014) Critical role for IL-1β in DNA damage-induced mucositis. Proc Natl Acad Sci U S A 111:E702-11

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