The mission of this research program is to apply our expertise in organic chemistry to the design and synthesis of novel anti-tubercular, antibiotic, anticancer, and steroid-based drug candidates. Each of the scientific aims of this program will seek chemical innovation in the pursuit of high profile, natural product-based targets.
In Aim I, we will develop a transformative new strategy for achieving rapid and flexible syntheses of diverse collections of steroid-based pharmaceutical candidates from simple precursor fragments. Our approach is designed to provide access to many new varieties of steroids, incorporating substitution patterns that have not yet been explored in biological research. The goal of Aim II is to respond to the rising threat to public health caused by multidrug resistant strains of Mycobacterium tuberculosis and the alarming lack of new antibiotic drug candidates. Through thoughtful molecular design and chemical synthesis, we will invent new candidates for antibiotic and antitubercular drug development efforts based on the tricyclic architecture that defines the pleuromutilin class of bacterial protein synthesis inhibitors. Notably, these candidates will be engineered to exhibit enhanced metabolic stability compared to the currently known members of the pleuromutilin family. We will probe the stability, antitubercular activity, and broad-spectrum antibiotic activity of our new pleuromutilin-based compounds through research collaborations.
In Aim III of this proposal, we will complete a short and innovative synthesis of the high profile natural product, maoecrystal V. Isolated in 2004, maoecrystal V has attracted significant attention from the biomedical community due to reports of its potent and selective cytotoxic activity against cervical cancer cells. We propose herein to employ the concepts and methods of chemical synthesis to gain access to significant quantities of the natural product for collaborative studies directed toward the elucidation of the mechanism of action of maoecrystal V. Our design for synthesis will also open new avenues by which to access structurally novel screening candidates incorporating key elements of the structure of maoecrystal V.

Public Health Relevance

Advances in chemical synthesis enable the design and production of structurally unique molecules that selectively modulate biological processes and have great potential as molecular therapeutics for the treatment of human diseases. Through innovative advances in chemical synthesis, this research program will create new antibiotic, anti-tubercular, anticancer, and steroid-based candidates for drug development efforts. The power of the approaches described herein lie in their ability to create not only known therapeutic agents, but also novel, specifically modified molecules with improved therapeutic properties.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM065483-10
Application #
8372646
Study Section
Synthetic and Biological Chemistry B Study Section (SBCB)
Program Officer
Lees, Robert G
Project Start
2003-05-01
Project End
2016-07-31
Budget Start
2012-08-01
Budget End
2013-07-31
Support Year
10
Fiscal Year
2012
Total Cost
$331,079
Indirect Cost
$116,079
Name
Princeton University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
002484665
City
Princeton
State
NJ
Country
United States
Zip Code
08544
Siler, David A; Mighion, Jeffrey D; Sorensen, Erik J (2014) An enantiospecific synthesis of jiadifenolide. Angew Chem Int Ed Engl 53:5332-5
Reber, Keith P; Tilley, S David; Carson, Cheryl A et al. (2013) Toward a synthesis of hirsutellone B by the concept of double cyclization. J Org Chem 78:9584-607
Xie, Hao; Sammis, Glenn M; Flamme, Eric M et al. (2011) The catalytic asymmetric Diels-Alder reactions and post-cycloaddition reductive transpositions of 1-hydrazinodienes. Chemistry 17:11131-4
Guerrero, Carlos A; Sorensen, Erik J (2011) Concise, stereocontrolled synthesis of the citrinadin B core architecture. Org Lett 13:5164-7
Lotesta, Stephen D; Liu, Junjia; Yates, Emma V et al. (2011) Expanding the pleuromutilin class of antibiotics by de novo chemical synthesis. Chem Sci 2:1258-1261
Liu, Junjia; Lotesta, Stephen D; Sorensen, Erik J (2011) A concise synthesis of the molecular framework of pleuromutilin. Chem Commun (Camb) 47:1500-2
Frie, Jessica L; Jeffrey, Christopher S; Sorensen, Erik J (2009) A hypervalent iodine-induced double annulation enables a concise synthesis of the pentacyclic core structure of the cortistatins. Org Lett 11:5394-7
Tilley, S David; Reber, Keith P; Sorensen, Erik J (2009) A rapid, asymmetric synthesis of the decahydrofluorene core of the hirsutellones. Org Lett 11:701-3
Shipe, William D; Sorensen, Erik J (2006) Convergent, enantioselective syntheses of guanacastepenes A and E featuring a selective cyclobutane fragmentation. J Am Chem Soc 128:7025-35
Drahl, Carmen; Cravatt, Benjamin F; Sorensen, Erik J (2005) Protein-reactive natural products. Angew Chem Int Ed Engl 44:5788-809

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