Signal transduction pathways rely on reversible chemical modifications to relay information within and across cells. Covalent modification of protein substrates by ubiquitin and the ubiquitin-like proteins such as SUMO (small ubiquitin-like modifier) contribute to pathways that regulate cellular functions including nuclear transport, cytokinesis, chromosome segregation, G2-M cell cycle progression and transcriptional regulation among many others. Post-translational modification by ubiquitin (Ub) and ubiquitin-like (Ubl) proteins requires the sequential action of E1 activating enzymes, E2 conjugating enzymes and E3 ligases while Ub/Ubl processing and deconjugation is catalyzed by Ub/Ubl-specific proteases. Ubiquitin and SUMO conjugation play an integral role in eukaryotic nuclear metabolism and cell cycle control and our studies are of direct relevance to human health, cancer, and the mission of the NIH. This proposal seeks to address the functional significance for components of the ubiquitin and SUMO conjugation pathways through structural, biochemical and genetic studies that will establish the basis for Ub/Ubl 1) activation, 2) conjugation by E2 and E3 enzymes, 3) signal transduction through characterization receptors that recognize Ub/Ubl-conjugated substrates. The enzymes, mechanisms and factors that constitute ubiquitin and SUMO protein conjugation pathways are conserved so our studies will be broadly relevant and will impact research in other Ub/Ubl-related pathways.

Public Health Relevance

Post-translational protein modification relays information within and across cells in signal transduction pathways that control the activities of protein substrates as well as their spatial and temporal distribution. Covalent modification of proteins by ubiquitin (Ub) and ubiquitin-like (Ubl) proteins such as SUMO (small ubiquitin-like modifier) and Nedd8 impact nearly all facets of cellular metabolism including cell cycle control, protein degradation, protein localization, nuclear transport, cytokinesis, chromosome segregation, and transcriptional regulation among many others. This proposal seeks to explore the mechanisms that underlie protein modification by ubiquitin and SUMO through structural, biochemical and genetic studies that will address 1) Ub/Ubl activation by the E1 enzyme, 2) SUMO conjugation by E2 and E3 enzymes, 3) structural analysis of PCNA, its modification by Ub/Ubl proteins, and its recognition by Srs2 and Eco1. The ubiquitin and SUMO pathways regulate many processes that are associated with human disease conditions including neurodegenerative disorders, cancer, and inflammation.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM065872-14
Application #
8702424
Study Section
Membrane Biology and Protein Processing (MBPP)
Program Officer
Gerratana, Barbara
Project Start
2002-07-01
Project End
2018-05-31
Budget Start
2014-06-01
Budget End
2015-05-31
Support Year
14
Fiscal Year
2014
Total Cost
$357,177
Indirect Cost
$156,177
Name
Sloan-Kettering Institute for Cancer Research
Department
Type
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10065
Streich Jr, Frederick C; Lima, Christopher D (2014) Structural and functional insights to ubiquitin-like protein conjugation. Annu Rev Biophys 43:357-79
O'Rourke, Jacqueline Gire; Gareau, Jaclyn R; Ochaba, Joseph et al. (2013) SUMO-2 and PIAS1 modulate insoluble mutant huntingtin protein accumulation. Cell Rep 4:362-75
Olsen, Shaun K; Lima, Christopher D (2013) Structure of a ubiquitin E1-E2 complex: insights to E1-E2 thioester transfer. Mol Cell 49:884-96
Armstrong, Anthony A; Mohideen, Firaz; Lima, Christopher D (2012) Recognition of SUMO-modified PCNA requires tandem receptor motifs in Srs2. Nature 483:59-63
Gareau, Jaclyn R; Reverter, David; Lima, Christopher D (2012) Determinants of small ubiquitin-like modifier 1 (SUMO1) protein specificity, E3 ligase, and SUMO-RanGAP1 binding activities of nucleoporin RanBP2. J Biol Chem 287:4740-51
Lu, Xuequan; Olsen, Shaun K; Capili, Allan D et al. (2010) Designed semisynthetic protein inhibitors of Ub/Ubl E1 activating enzymes. J Am Chem Soc 132:1748-9
Gareau, Jaclyn R; Lima, Christopher D (2010) The SUMO pathway: emerging mechanisms that shape specificity, conjugation and recognition. Nat Rev Mol Cell Biol 11:861-71
Olsen, Shaun K; Capili, Allan D; Lu, Xuequan et al. (2010) Active site remodelling accompanies thioester bond formation in the SUMO E1. Nature 463:906-12
Mohideen, Firaz; Capili, Allan D; Bilimoria, Parizad M et al. (2009) A molecular basis for phosphorylation-dependent SUMO conjugation by the E2 UBC9. Nat Struct Mol Biol 16:945-52
Yunus, Ali A; Lima, Christopher D (2009) Structure of the Siz/PIAS SUMO E3 ligase Siz1 and determinants required for SUMO modification of PCNA. Mol Cell 35:669-82

Showing the most recent 10 out of 26 publications