The "chemical nose/tongue" approach presents a potential alternative to specific recognition and separations techniques. In this approach a sensor array is generated to provide differential interaction with analytes via selective receptors, generating a stimulus response pattern that can be statistically analyzed and used for the identification of individual target analytes and also analysis of complex mixtures. Recently, we have developed nanoparticle-fluorescent polymer sensors for identification of proteins, bacteria, and cancerous cells through a fluorophore-displacement mechanism as well as a highly sensitive nanoparticle-GFP based "chemical nose" strategy for protein detection in biofluid. Current chemical nose sensors for proteins and cell-surfaces are single channel, meaning a separate well or channel is required for each sensing element. This requirement for spatially distinct sensor elements complicates both microplate-based techniques and the application of array-based sensing in other venues, including microfluidics platforms and tissue staining. To overcome this limitation, we will exploit the spectral range of fluorescent proteins (FPs) to provide multi-channel fluorescence transduction for sensing applications. Multi-channel sensing will facilitate implementation of array-based sensing, allowing "one well" sensing while improving sensitivity through generation of ratiometric dadt. In this program, we will use the tools of supramolecular chemistry to synergistically engineer both the protein and AuNP quencher to provide highly efficient and versatile platforms for protein and cell surface sensing.

Public Health Relevance

Rapid and efficient sensing of proteins and cell surfaces will provide access to new diagnostics for many diseases.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM077173-06
Application #
8245843
Study Section
Nanotechnology Study Section (NANO)
Program Officer
Fabian, Miles
Project Start
2006-03-01
Project End
2015-03-31
Budget Start
2012-04-01
Budget End
2013-03-31
Support Year
6
Fiscal Year
2012
Total Cost
$293,756
Indirect Cost
$102,756
Name
University of Massachusetts Amherst
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
153926712
City
Amherst
State
MA
Country
United States
Zip Code
01003
Mout, Rubul; Tonga, Gulen Yesilbag; Ray, Moumita et al. (2014) Environmentally responsive histidine-carboxylate zipper formation between proteins and nanoparticles. Nanoscale 6:8873-7
Le, Ngoc D B; Yazdani, Mahdieh; Rotello, Vincent M (2014) Array-based sensing using nanoparticles: an alternative approach for cancer diagnostics. Nanomedicine (Lond) 9:1487-98
Scaletti, Federica; Kim, Chang Soo; Messori, Luigi et al. (2014) Rapid purification of gold nanorods for biomedical applications. MethodsX 1:118-123
Tonga, Gulen Yesilbag; Saha, Krishnendu; Rotello, Vincent M (2014) 25th anniversary article: interfacing nanoparticles and biology: new strategies for biomedicine. Adv Mater 26:359-70
Chen, Kaimin; Rana, Subinoy; Moyano, Daniel F et al. (2014) Optimizing the selective recognition of protein isoforms through tuning of nanoparticle hydrophobicity. Nanoscale 6:6492-5
Tang, Rui; Moyano, Daniel F; Subramani, Chandramouleeswaran et al. (2014) Rapid coating of surfaces with functionalized nanoparticles for regulation of cell behavior. Adv Mater 26:3310-4
Moyano, Daniel F; Rotello, Vincent M (2014) Gold nanoparticles: testbeds for engineered protein-particle interactions. Nanomedicine (Lond) 9:1905-7
Yeh, Yi-Cheun; Tang, Rui; Mout, Rubul et al. (2014) Fabrication of multiresponsive bioactive nanocapsules through orthogonal self-assembly. Angew Chem Int Ed Engl 53:5137-41
Tonga, Gulen Yesilbag; Moyano, Daniel F; Kim, Chang Soo et al. (2014) Inorganic Nanoparticles for Therapeutic Delivery: Trials, Tribulations and Promise. Curr Opin Colloid Interface Sci 19:49-55
Moyano, Daniel F; Duncan, Bradley; Rotello, Vincent M (2013) Preparation of 2 nm gold nanoparticles for in vitro and in vivo applications. Methods Mol Biol 1025:3-8

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