The """"""""chemical nose/tongue"""""""" approach presents a potential alternative to specific recognition and separations techniques. In this approach a sensor array is generated to provide differential interaction with analytes via selective receptors, generating a stimulus response pattern that can be statistically analyzed and used for the identification of individual target analytes and also analysis of complex mixtures. Recently, we have developed nanoparticle-fluorescent polymer sensors for identification of proteins, bacteria, and cancerous cells through a fluorophore-displacement mechanism as well as a highly sensitive nanoparticle-GFP based """"""""chemical nose"""""""" strategy for protein detection in biofluid. Current chemical nose sensors for proteins and cell-surfaces are single channel, meaning a separate well or channel is required for each sensing element. This requirement for spatially distinct sensor elements complicates both microplate-based techniques and the application of array-based sensing in other venues, including microfluidics platforms and tissue staining. To overcome this limitation, we will exploit the spectral range of fluorescent proteins (FPs) to provide multi-channel fluorescence transduction for sensing applications. Multi-channel sensing will facilitate implementation of array-based sensing, allowing """"""""one well"""""""" sensing while improving sensitivity through generation of ratiometric dadt. In this program, we will use the tools of supramolecular chemistry to synergistically engineer both the protein and AuNP quencher to provide highly efficient and versatile platforms for protein and cell surface sensing.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM077173-08
Application #
8636480
Study Section
Nanotechnology Study Section (NANO)
Program Officer
Fabian, Miles
Project Start
2007-03-08
Project End
2015-03-31
Budget Start
2014-04-01
Budget End
2015-03-31
Support Year
8
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Massachusetts Amherst
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
City
Amherst
State
MA
Country
United States
Zip Code
01003
Mout, Rubul; Rotello, Vincent M (2017) A General Method for Intracellular Protein Delivery through 'E-tag' Protein Engineering and Arginine Functionalized Gold Nanoparticles. Bio Protoc 7:
Han, Jinsong; Wang, Benhua; Bender, Markus et al. (2017) Fingerprinting antibiotics with PAE-based fluorescent sensor arrays. Polym Chem 8:2723-2732
Mout, Rubul; Ray, Moumita; Tay, Tristan et al. (2017) General Strategy for Direct Cytosolic Protein Delivery via Protein-Nanoparticle Co-engineering. ACS Nano 11:6416-6421
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Ray, Moumita; Lee, Yi-Wei; Scaletti, Federica et al. (2017) Intracellular delivery of proteins by nanocarriers. Nanomedicine (Lond) 12:941-952
Mout, Rubul; Rotello, Vincent M (2017) Cytosolic and Nuclear Delivery of CRISPR/Cas9-ribonucleoprotein for Gene Editing Using Arginine Functionalized Gold Nanoparticles. Bio Protoc 7:
Le, Ngoc D B; Wang, Xian; Geng, Yingying et al. (2017) Rapid and ultrasensitive detection of endocrine disrupting chemicals using a nanosensor-enabled cell-based platform. Chem Commun (Camb) 53:8794-8797
Elci, S Gokhan; Yesilbag Tonga, Gulen; Yan, Bo et al. (2017) Dual-Mode Mass Spectrometric Imaging for Determination of in Vivo Stability of Nanoparticle Monolayers. ACS Nano 11:7424-7430

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