Evolutionary forces are known to drive the fixation of locally adaptive genetic variation within populations, and such variation is thought to be involved in the differential response to disease and pharmacological agents observed in human populations. Natural variation is well documented in plants, and plants provide an excellent model for investigations into its genetic origins, maintenance and adaptive significance.
We aim to take advantage of the natural genetic variation that occurs between populations of the genetic model plant Arabidopsis thaliana (Arabidopsis) to identify gene functions that vary between these natural populations. Such a set of polymorphic genes and gene functions will provide the essential tools required in the future to uncover the genetic mechanisms that drive the fixation of locally adaptive genetic variation within a population. To link natural genetic variation to function, we will apply our high-throughput Inductively Coupled Plasma - Mass Spectroscopy (ICP-MS) based elemental-profiling platform, in combination with powerful genetic mapping tools, to a genotyped panel of 1000 Arabidopsis accessions and Recombinant Inbred Lines (RILs). Such a system will allow the identification of loci that drive the natural variation we observe in the plant's elemental-profile or """"""""ionome"""""""" including P, Ca, K, Mg (macronutrients);Cu, Fe, Zn, Mn, Co, Ni, Se, Mo, I (micronutrients of significance to plant and human health);Na, As, and Cd (minerals causing agricultural or environmental problems). We have demonstrated the feasibility of such studies by identifying naturally occurring alleles of several genes in Arabidopsis that function to control shoot levels of various elements, including Na, Co and Mo. Sequence analysis of the haplotypes of such loci across the large panel of Arabidopsis populations will identify which of these genes have been under recent directional selection due to local adaptation. Such information will help shed light on the evolutionary processes involved in adaptation of organisms to local environments, to understanding ion homeostasis networks and will have applications to improving the mineral content of food crops for improved human health. After all, plants provide the major source of nutrition for a large portion of the world's population.
|Wang, Chengcheng; Na, GunNam; Bermejo, Eduardo Sanchez et al. (2018) Dissecting the components controlling root-to-shoot arsenic translocation in Arabidopsis thaliana. New Phytol 217:206-218|
|Chu, Heng-Hsuan; Car, Suzana; Socha, Amanda L et al. (2017) The Arabidopsis MTP8 transporter determines the localization of manganese and iron in seeds. Sci Rep 7:11024|
|Hindt, Maria N; Akmakjian, Garo Z; Pivarski, Kara L et al. (2017) BRUTUS and its paralogs, BTS LIKE1 and BTS LIKE2, encode important negative regulators of the iron deficiency response in Arabidopsis thaliana. Metallomics 9:876-890|
|Shi, Shulin; Wang, Tao; Chen, Ziru et al. (2016) OsHAC1;1 and OsHAC1;2 Function as Arsenate Reductases and Regulate Arsenic Accumulation. Plant Physiol 172:1708-1719|
|Arnold, Brian J; Lahner, Brett; DaCosta, Jeffrey M et al. (2016) Borrowed alleles and convergence in serpentine adaptation. Proc Natl Acad Sci U S A 113:8320-5|
|Guerinot, Mary Lou (2016) Micronutrients: Supplementing seeds with zinc. Nat Plants 2:16060|
|Huang, Xin-Yuan; Salt, David E (2016) Plant Ionomics: From Elemental Profiling to Environmental Adaptation. Mol Plant 9:787-97|
|Forsberg, Simon K G; Andreatta, Matthew E; Huang, Xin-Yuan et al. (2015) The Multi-allelic Genetic Architecture of a Variance-Heterogeneity Locus for Molybdenum Concentration in Leaves Acts as a Source of Unexplained Additive Genetic Variance. PLoS Genet 11:e1005648|
|Busoms, Silvia; Teres, Joana; Huang, Xin-Yuan et al. (2015) Salinity Is an Agent of Divergent Selection Driving Local Adaptation of Arabidopsis to Coastal Habitats. Plant Physiol 168:915-29|
|Chao, Dai-Yin; Baraniecka, Patrycja; Danku, John et al. (2014) Variation in sulfur and selenium accumulation is controlled by naturally occurring isoforms of the key sulfur assimilation enzyme ADENOSINE 5'-PHOSPHOSULFATE REDUCTASE2 across the Arabidopsis species range. Plant Physiol 166:1593-608|
Showing the most recent 10 out of 31 publications