Proper spatial and temporal regulation of the cell cycle is crucial to the survival and proliferation of all organisms. Complex regulatory circuits have evolved to ensure stable, orderly progression through the cell cycle. Surprisingly, the molecular mechanisms and design principles underlying these circuits remain incompletely understood, particularly in bacteria. The alpha-proteobacterium Caulobacter crescentus is an experimentally tractable system for elucidating the fundamental mechanisms underlying cell cycle regulation in bacteria, and for understanding regulation at the systems level. The Caulobacter cell cycle is driven largely by two essential regulators: CtrA and DnaA. CtrA is a response regulator and two-component signal transduction protein that directly regulates the expression of nearly 100 genes, many of which coordinate cell division. CtrA also directly binds to and silences the origin of replication in daughter swarmer cells, but is inactivated and degraded in daughter stalked cells. CtrA thus governs the asymmetric replicative fates of daughter cells, a hallmark of the Caulobacter cell cycle. However, CtrA does not significantly influence the fundamental periodicity of DNA replication. Instead, DnaA, a nearly universal bacterial protein that initiates DNA replication, governs the periodicity of replication. In rich media, DnaA protein levels are constant, indicating that it is regulated predominantly at the level of activity. Although substantial progress has been made in understanding the regulation of CtrA and, to a lesser extent, DnaA, major gaps remain. The goal of this project is to define the complete molecular circuitry controlling CtrA and DnaA in both nutrient replete conditions and following starvation.
We aim to identify new components of this circuitry, to elucidate their connections to DnaA and CtrA, and to understand their dynamics using a combination of genetic, biochemical, bioinformatic, genomic, and cell biological approaches. Specifically, we aim to (i) determine how the activation of CtrA is coupled to DNA replication initiation, (ii) idenify and characterize factors that regulate DnaA activity during growth in rich media, and (iii) elucidate the molecular mechanisms governing CtrA and DnaA activity following nutrient starvation. These studies will unveil how bacterial cells tightly regulate DNA replication and modulate cell cycle progression in a range of growth conditions. A better understanding of how bacteria regulate their cell cycle will guide the development of new antibiotics that slow or halt the proliferation of pathogens. Finally, by comparing the regulatory strategies unveiled here to those employed in eukaryotes our work will help to reveal the design principles of regulatory circuits throughout biology.

Public Health Relevance

This project will aid efforts to develop new antibiotics by identifying and understanding essential components of the cell cycle machinery in bacteria. We have a specific focus on two-component signal transduction proteins, which have emerged as attractive drug targets because they are critical to the proliferation of bacteria but conspicuousl absent from metazoans. We are also focused on understanding DnaA, a nearly universal regulator of DNA replication in bacteria that is also, in principle, an ideal target for new antibiotics.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Research Project (R01)
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Prokaryotic Cell and Molecular Biology Study Section (PCMB)
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Hamlet, Michelle R
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Massachusetts Institute of Technology
Schools of Arts and Sciences
United States
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Le, Tung Bk; Laub, Michael T (2014) New approaches to understanding the spatial organization of bacterial genomes. Curr Opin Microbiol 22:15-21
Modell, Joshua W; Kambara, Tracy K; Perchuk, Barrett S et al. (2014) A DNA damage-induced, SOS-independent checkpoint regulates cell division in Caulobacter crescentus. PLoS Biol 12:e1001977
Le, Tung B K; Imakaev, Maxim V; Mirny, Leonid A et al. (2013) High-resolution mapping of the spatial organization of a bacterial chromosome. Science 342:731-4
Gora, Kasia G; Cantin, Amber; Wohlever, Matthew et al. (2013) Regulated proteolysis of a transcription factor complex is critical to cell cycle progression in Caulobacter crescentus. Mol Microbiol 87:1277-89
Aakre, Christopher D; Phung, Tuyen N; Huang, David et al. (2013) A bacterial toxin inhibits DNA replication elongation through a direct interaction with the * sliding clamp. Mol Cell 52:617-28
Jonas, Kristina; Liu, Jing; Chien, Peter et al. (2013) Proteotoxic stress induces a cell-cycle arrest by stimulating Lon to degrade the replication initiator DnaA. Cell 154:623-36
Podgornaia, Anna I; Casino, Patricia; Marina, Alberto et al. (2013) Structural basis of a rationally rewired protein-protein interface critical to bacterial signaling. Structure 21:1636-47
Chen, Y Erin; Tropini, Carolina; Jonas, Kristina et al. (2011) Spatial gradient of protein phosphorylation underlies replicative asymmetry in a bacterium. Proc Natl Acad Sci U S A 108:1052-7
Tsokos, Christos G; Perchuk, Barrett S; Laub, Michael T (2011) A dynamic complex of signaling proteins uses polar localization to regulate cell-fate asymmetry in Caulobacter crescentus. Dev Cell 20:329-41
Jonas, Kristina; Chen, Y Erin; Laub, Michael T (2011) Modularity of the bacterial cell cycle enables independent spatial and temporal control of DNA replication. Curr Biol 21:1092-101

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