In this proposal we aim to define the molecular mechanisms by which membrane-bound organelles are organized in magnetotactic bacteria. These microorganisms are capable of using the earth's magnetic field as a guide to simplify their search for low oxygen environments with the help of a specialized organelle termed the magnetosome. Magnetosomes are small lipid-bilayer invagination of the inner cell membrane within which nanometer-sized iron-based magnetic particles are produced. Individual magnetosomes are arranged into a chain that maximizes the interaction of the bacterium with magnetic fields. The work of many groups, including ours, has pinpointed a large number of genes as having specific roles in building the magnetosome organelle. One of these factors, MamK, is a bacterial actin-like protein that is at the center of magnetosome chain formation. In the absence of MamK, magnetosomes fail to form coherent chains and are instead separated by numerous gaps. Bacterial actins are widespread amongst the Bacteria and form numerous families with distinct functions. For instance, MreB is involved in directing the synthesis of the cell wall whereas ParM and other actins help in segregation of naturally occurring plasmids. Outside of MreB and ParM, little is known regarding the function and behavior of the vast majority of the bacterial actin-like proteins. MamK is the founding member of one of these families and its representatives are mostly found within the MB. With the support of this grant over the last four years, we have developed an in vitro system to study the polymerization kinetics of MamK and, through collaborations, obtained a high-resolution electron microscopic structure of its filaments. Furthermore, we identified two proteins, MamJ and LimJ that are responsible for the dynamic turnover of MamK filaments in vivo. Using a directed mutagenesis strategy, we have also identified intrinsic features of MamK that contribute to its function, localization, bundling and dynamics. Finally, we have used broader genetic strategies to define the functions of over 20 genes in the formation of magnetosomes and biomineralization of magnetic particles. In this proposal, we have will build on our exclusive expertise and infrastructure to define the mechanisms of MamK function and magnetosome formation in more detail. First, we will ask if the biochemical and structural features of MamK are represented in the diverse members of its family, some of which exist in non-magnetotactic bacteria as well as the archaea. Second, we will define and characterize regulators and interactors of MamK. Third, we will develop tools to ask if MamK is involved in chain establishment, maintenance or segregation. Finally, we will develop tools to image and understand the earliest steps of magnetosome membrane biogenesis. These experiments promise to shed mechanistic light on the cell biology of bacterial organelles and the evolution and functional diversity of bacterial actins.

Public Health Relevance

Here we use the magnetosome compartments of magnetotactic bacteria as a general model to understand subcellular differentiation and magnetic particle formation in bacterial cells. By focusing on the MamK protein, we hope to learn about the general features of bacterial actin-like proteins, some of which are potential antibiotic targets in pathogenic microorganisms. Moreover, by understanding magnetosome formation in detail, we will be able to design novel applications that exploit their unique magnetic properties.

Agency
National Institute of Health (NIH)
Type
Research Project (R01)
Project #
2R01GM084122-06
Application #
8697647
Study Section
Prokaryotic Cell and Molecular Biology Study Section (PCMB)
Program Officer
Deatherage, James F
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of California Berkeley
Department
Other Basic Sciences
Type
Earth Sciences/Resources
DUNS #
City
Berkeley
State
CA
Country
United States
Zip Code
94704
Cornejo, Elias; Subramanian, Poorna; Li, Zhuo et al. (2016) Dynamic Remodeling of the Magnetosome Membrane Is Triggered by the Initiation of Biomineralization. MBio 7:e01898-15
Hershey, David M; Browne, Patrick J; Iavarone, Anthony T et al. (2016) Magnetite Biomineralization in Magnetospirillum magneticum Is Regulated by a Switch-like Behavior in the HtrA Protease MamE. J Biol Chem 291:17941-52
Hershey, David M; Ren, Xuefeng; Melnyk, Ryan A et al. (2016) MamO Is a Repurposed Serine Protease that Promotes Magnetite Biomineralization through Direct Transition Metal Binding in Magnetotactic Bacteria. PLoS Biol 14:e1002402
Rahn-Lee, Lilah; Byrne, Meghan E; Zhang, Manjing et al. (2015) A genetic strategy for probing the functional diversity of magnetosome formation. PLoS Genet 11:e1004811
Jones, Stephanie R; Wilson, Tiffany D; Brown, Margaret E et al. (2015) Genetic and biochemical investigations of the role of MamP in redox control of iron biomineralization in Magnetospirillum magneticum. Proc Natl Acad Sci U S A 112:3904-9
Chariaou, Michalis; Rahn-Lee, Lilah; Kind, Jessica et al. (2015) Anisotropy of bullet-shaped magnetite nanoparticles in the magnetotactic bacteria Desulfovibrio magneticus sp. Strain RS-1. Biophys J 108:1268-74
Cornejo, Elias; Abreu, Nicole; Komeili, Arash (2014) Compartmentalization and organelle formation in bacteria. Curr Opin Cell Biol 26:132-8
Abreu, Nicole; Mannoubi, Soumaya; Ozyamak, Ertan et al. (2014) Interplay between two bacterial actin homologs, MamK and MamK-Like, is required for the alignment of magnetosome organelles in Magnetospirillum magneticum AMB-1. J Bacteriol 196:3111-21
Ozyamak, Ertan; Kollman, Justin; Agard, David A et al. (2013) The bacterial actin MamK: in vitro assembly behavior and filament architecture. J Biol Chem 288:4265-77
Le Sage, D; Arai, K; Glenn, D R et al. (2013) Optical magnetic imaging of living cells. Nature 496:486-9

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