Research Narrative: This project will provide a powerful new tool for molecular, cellular, and diagnostic imaging by allowing researchers to light up whatever cells they are interested in. Adding a bit of DNA to a cell will give it the ability to make its own luminescence. The possible applications are nearly limitless, but include making tumors reveal themselves by glowing, detecting contamination by causing pathogens to emit light, and following the time course of gene expression in organisms.
|Francis, Warren R; Christianson, Lynne M; Powers, Meghan L et al. (2016) Non-excitable fluorescent protein orthologs found in ctenophores. BMC Evol Biol 16:167|
|Francis, Warren R; Shaner, Nathan C; Christianson, Lynne M et al. (2015) Occurrence of Isopenicillin-N-Synthase Homologs in Bioluminescent Ctenophores and Implications for Coelenterazine Biosynthesis. PLoS One 10:e0128742|
|Francis, Warren R; Powers, Meghan L; Haddock, Steven H D (2014) Characterization of an anthraquinone fluor from the bioluminescent, pelagic polychaete Tomopteris. Luminescence 29:1135-40|
|Powers, Meghan L; McDermott, Amy G; Shaner, Nathan C et al. (2013) Expression and characterization of the calcium-activated photoprotein from the ctenophore Bathocyroe fosteri: insights into light-sensitive photoproteins. Biochem Biophys Res Commun 431:360-6|
|Schnitzler, Christine E; Pang, Kevin; Powers, Meghan L et al. (2012) Genomic organization, evolution, and expression of photoprotein and opsin genes in Mnemiopsis leidyi: a new view of ctenophore photocytes. BMC Biol 10:107|
|Thuesen, Erik V; Goetz, Freya E; Haddock, Steven H D (2010) Bioluminescent organs of two deep-sea arrow worms, Eukrohnia fowleri and Caecosagitta macrocephala, with further observations on Bioluminescence in chaetognaths. Biol Bull 219:100-11|
|Chudakov, Dmitriy M; Matz, Mikhail V; Lukyanov, Sergey et al. (2010) Fluorescent proteins and their applications in imaging living cells and tissues. Physiol Rev 90:1103-63|