We will utilize polymeric phospholipid membranes to prepare a new generation of biochemical separation matrices that exhibit marked improvements in selectivity, stability, reproducibility and tenability. We will focus on two primary objectives: design and implementation of a) membrane protein functionalized stationary phase materials and b) ion channel functionalized detectors for microchip separations. To achieve these goals, silica particles (ranging in diameter from 0.5 to 5

Public Health Relevance

This research project will develop and implement new, state-of-the-art technologies for biochemical separations, allowing for identification of novel pharmacological and physiological regulators of biological function. This research project will also provide key enabling technologies that lead to a more efficient elucidation of key biochemical and biophysical parameters for the design of next generation peptide-based drugs for treatment of a range of disease states.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM095763-03
Application #
8465243
Study Section
Enabling Bioanalytical and Imaging Technologies Study Section (EBIT)
Program Officer
Edmonds, Charles G
Project Start
2011-05-01
Project End
2015-04-30
Budget Start
2013-05-01
Budget End
2014-04-30
Support Year
3
Fiscal Year
2013
Total Cost
$248,217
Indirect Cost
$79,342
Name
University of Arizona
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
806345617
City
Tucson
State
AZ
Country
United States
Zip Code
85721
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Baker, Christopher A; Aspinwall, Craig A (2015) Emerging trends in precision fabrication of microapertures to support suspended lipid membranes for sensors, sequencing, and beyond. Anal Bioanal Chem 407:647-52
Gallagher, Elyssia S; Adem, Seid M; Bright, Leonard K et al. (2014) Hybrid phospholipid bilayer coatings for separations of cationic proteins in capillary zone electrophoresis. Electrophoresis 35:1099-105
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Baker, Christopher A; Bright, Leonard K; Aspinwall, Craig A (2013) Photolithographic fabrication of microapertures with well-defined, three-dimensional geometries for suspended lipid membrane studies. Anal Chem 85:9078-86
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Adem, Seid M; Mansfield, Elisabeth; Keogh, John P et al. (2013) Practical considerations for preparing polymerized phospholipid bilayer capillary coatings for protein separations. Anal Chim Acta 772:93-8