We will utilize polymeric phospholipid membranes to prepare a new generation of biochemical separation matrices that exhibit marked improvements in selectivity, stability, reproducibility and tenability. We will focus on two primary objectives: design and implementation of a) membrane protein functionalized stationary phase materials and b) ion channel functionalized detectors for microchip separations. To achieve these goals, silica particles (ranging in diameter from 0.5 to 5

Public Health Relevance

This research project will develop and implement new, state-of-the-art technologies for biochemical separations, allowing for identification of novel pharmacological and physiological regulators of biological function. This research project will also provide key enabling technologies that lead to a more efficient elucidation of key biochemical and biophysical parameters for the design of next generation peptide-based drugs for treatment of a range of disease states.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM095763-03
Application #
8465243
Study Section
Enabling Bioanalytical and Imaging Technologies Study Section (EBIT)
Program Officer
Edmonds, Charles G
Project Start
2011-05-01
Project End
2015-04-30
Budget Start
2013-05-01
Budget End
2014-04-30
Support Year
3
Fiscal Year
2013
Total Cost
$248,217
Indirect Cost
$79,342
Name
University of Arizona
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
806345617
City
Tucson
State
AZ
Country
United States
Zip Code
85721
Bright, Leonard K; Baker, Christopher A; Bränström, Robert et al. (2015) Methacrylate Polymer Scaffolding Enhances the Stability of Suspended Lipid Bilayers for Ion Channel Recordings and Biosensor Development. ACS Biomater Sci Eng 1:955-963
Li, Zhen; Muhandiramlage, Thusitha P; Keogh, John P et al. (2015) Aptamer-functionalized porous phospholipid nanoshells for direct measurement of Hg(2+) in urine. Anal Bioanal Chem 407:953-60
Gallagher, Elyssia S; Adem, Seid M; Baker, Christopher A et al. (2015) Highly stabilized, polymer-lipid membranes prepared on silica microparticles as stationary phases for capillary chromatography. J Chromatogr A 1385:28-34
Baker, Christopher A; Aspinwall, Craig A (2015) Emerging trends in precision fabrication of microapertures to support suspended lipid membranes for sensors, sequencing, and beyond. Anal Bioanal Chem 407:647-52
Gallagher, Elyssia S; Adem, Seid M; Bright, Leonard K et al. (2014) Hybrid phospholipid bilayer coatings for separations of cationic proteins in capillary zone electrophoresis. Electrophoresis 35:1099-105
Cheng, Zhiliang; Al Zaki, Ajlan; Jones, Ian W et al. (2014) Stabilized porous liposomes with encapsulated Gd-labeled dextran as a highly efficient MRI contrast agent. Chem Commun (Camb) 50:2502-4
Johnson, Gail M; Chozinski, Tyler J; Gallagher, Elyssia S et al. (2014) Glutathione sulfinamide serves as a selective, endogenous biomarker for nitroxyl after exposure to therapeutic levels of donors. Free Radic Biol Med 76:299-307
Gallagher, Elyssia S; Mansfield, Elisabeth; Aspinwall, Craig A (2014) Stabilized phospholipid membranes in chromatography: toward membrane protein-functionalized stationary phases. Anal Bioanal Chem 406:2223-9
Zhou, Yi; Bright, Leonard K; Shi, Wenqing et al. (2014) Ion channel probes for scanning ion conductance microscopy. Langmuir 30:15351-5
Bright, Leonard K; Baker, Christopher A; Agasid, Mark T et al. (2013) Decreased aperture surface energy enhances electrical, mechanical, and temporal stability of suspended lipid membranes. ACS Appl Mater Interfaces 5:11918-26

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