We propose label-free high-throughput detection of cells response to chemicals by merging microwell microtechnology with state-of-the-art label-free biosensing technology based on optical microresonators. Arrays of optical microbead sensors will be sealed against arrays of cells in microwells to monitor secretion of protein in response to chemicals released from a hydrogel. The platform can be operated on a conventional total-internal-reflection microscope setup.

Public Health Relevance

Cells in microwell arrays fabricated from polyethyleneglycol polymer will be sealed against an array of optical microbead biosensors co-localized with a hydrogel array for controlled release of chemicals. Secreted apoptotic biomarkers such as caspase-3 protein will be monitored in label-free manner and in real-time in response to toxic chemicals released from the hydrogels.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM095906-04
Application #
8735956
Study Section
Instrumentation and Systems Development Study Section (ISD)
Program Officer
Edmonds, Charles G
Project Start
2011-09-19
Project End
2016-08-31
Budget Start
2014-09-01
Budget End
2015-08-31
Support Year
4
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Brigham and Women's Hospital
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02115
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