Monoclonal antibodies (MAbs) of the immunoglobulin G (IgG) type are an important class of therapeutic glycoproteins. Compelling evidence has indicated that the fine structures of the glycans at the conserved N-glycosylation site (Asn-297) of the Fc domain are responsible for the distinct effector functions of MAbs, including antibody-dependent cellular cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC), and activation of apoptosis. In addition, a special sialylated Fc glycoform was identified to be responsible for the antiinflammatory activity of intravenous immunoglobulin (IVIG). However, progress in understanding the functional roles of IgG-Fc glycosylation is hampered by the tremendous structural heterogeneity of Fc domain glycans. In addition, controlling glycosylation of MAbs in expression to a desired homogeneous glycoform is still a challenging task. In this application, we propose to explore a chemoenzymatic method to make a library of homogeneously glycosylated IgG-Fc and selected glycoforms of MAbs. Through performing Fc receptor binding studies, we aim to understand how different glycan structures can fine tune the effector functions of IgG and IgG-Fc protein. We have performed important preliminary studies indicating that it is feasible to use the endoglycosidase-based transglycosylation approach to construct defined, homogeneous glycoforms of human IgG-Fc. Building on this success, we propose to pursue three specific aims.
Aim 1 is to explore a chemoenzymatic method for the construction of various pure glycoforms of IgG-Fc.
Aim 2 is to evaluate the structure-activity realtionships of different Fc domain glycoforms in Fc receptor binding, and to evaluate ADCC activity of selectively glycoengineered monoclonal antibodies.
Aim 3 is to synthesize novel Fc domain glycoforms for evaluating the roles of IgG-Fc glycosylation in anti-inflammatory activity. The knowledge gained from the proposed research will eventually facilitate the development of novel glycoforms of MAbs and IgG-Fc proteins as effective therapeutics.

Public Health Relevance

IgG antibodies are an important class of therapeutic glycoproteins. The Fc domain glycosylation is essential for antibody's effector functions including ADCC and anti-inflammatory activity. The proposed research aims to decipher the functional roles of Fc glycosylation through glycosylation engineering and Fc receptor binding studies, which may lead to the discovery of novel antibody glycoforms with potent therapeutic efficacy.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM096973-03
Application #
8445404
Study Section
Special Emphasis Panel (ZRG1-BCMB-B (03))
Program Officer
Marino, Pamela
Project Start
2011-07-01
Project End
2015-03-31
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
3
Fiscal Year
2013
Total Cost
$289,500
Indirect Cost
$96,500
Name
University of Maryland Baltimore
Department
Biochemistry
Type
Schools of Medicine
DUNS #
188435911
City
Baltimore
State
MD
Country
United States
Zip Code
21201
Li, Tiezheng; Li, Chao; Quan, David N et al. (2018) Site-specific immobilization of endoglycosidases for streamlined chemoenzymatic glycan remodeling of antibodies. Carbohydr Res 458-459:77-84
Giddens, John P; Lomino, Joseph V; DiLillo, David J et al. (2018) Site-selective chemoenzymatic glycoengineering of Fab and Fc glycans of a therapeutic antibody. Proc Natl Acad Sci U S A 115:12023-12027
Li, Chao; Wang, Lai-Xi (2018) Chemoenzymatic Methods for the Synthesis of Glycoproteins. Chem Rev 118:8359-8413
Bennett, Lindsay D; Yang, Qiang; Berquist, Brian R et al. (2018) Implementation of Glycan Remodeling to Plant-Made Therapeutic Antibodies. Int J Mol Sci 19:
Tong, Xin; Li, Tiezheng; Orwenyo, Jared et al. (2018) One-pot enzymatic glycan remodeling of a therapeutic monoclonal antibody by endoglycosidase S (Endo-S) from Streptococcus pyogenes. Bioorg Med Chem 26:1347-1355
Li, Chao; Li, Tiezheng; Wang, Lai-Xi (2018) Chemoenzymatic Defucosylation of Therapeutic Antibodies for Enhanced Effector Functions Using Bacterial ?-Fucosidases. Methods Mol Biol 1827:367-380
Tong, Xin; Li, Tiezheng; Li, Chao et al. (2018) Generation and Comparative Kinetic Analysis of New Glycosynthase Mutants from Streptococcus pyogenes Endoglycosidases for Antibody Glycoengineering. Biochemistry 57:5239-5246
Li, Tiezheng; DiLillo, David J; Bournazos, Stylianos et al. (2017) Modulating IgG effector function by Fc glycan engineering. Proc Natl Acad Sci U S A 114:3485-3490
Yang, Qiang; Wang, Lai-Xi (2017) Chemoenzymatic Glycan Remodeling of Natural and Recombinant Glycoproteins. Methods Enzymol 597:265-281
Li, Chao; Zhu, Shilei; Ma, Christopher et al. (2017) Designer ?1,6-Fucosidase Mutants Enable Direct Core Fucosylation of Intact N-Glycopeptides and N-Glycoproteins. J Am Chem Soc 139:15074-15087

Showing the most recent 10 out of 23 publications