The goal of the proposed collaborative research is to understand how distinct social behaviors are encoded in the genome and wired in the brain. Using a novel natural vertebrate system we will trace key behaviors to their genomic roots. Specifically, we will study innate 'bower' (reproductive nest) construction by male cichlid fishes from Lake Malawi, East Africa. This behavior is an essential part of social and reproductive interactions between males and females.
In Aim 1, we will quantify the elements of bower behavior, comparing pit-digging vs. castle-building cichlid species and their F1 hybrids.
Aim 2 will map brain regions recruited during male bower behavior by visualizing the expression of "immediate early" genes that reflect neural activity. Transcriptional divergence in these focal brain regions will then be quantified by allele-specific expression analysis in F1 hybrid males, who construct bowers in a phased sequence of species-specific behaviors.
Aim 3 will identify regions of the genome responsible for differences in bower behavior by re-sequencing the genomes of bulked F2 individuals who either dig pits or build castles. Our integrative approach is designed to solve problems that are currently impossible, or at best difficult to study in standard inbred model organisms. Our strategy to identify the genetic control of neural circuits regulating essential behaviors in outbred vertebrates will provide a natural model of human behavioral diversity.

Public Health Relevance

This research project asks how neural circuits controlling vertebrate behavior are genetically encoded. Such knowledge is central to a comprehensive understanding of neurological disorders and diseases of the brain, which affect one in every seven humans.

Agency
National Institute of Health (NIH)
Type
Research Project (R01)
Project #
1R01GM101095-01A1
Application #
8631179
Study Section
Genetic Variation and Evolution Study Section (GVE)
Program Officer
Sesma, Michael A
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Georgia Institute of Technology
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
City
Atlanta
State
GA
Country
United States
Zip Code
30332