Proteomics has special significance for Xenopus research because key events in meiosis, fertilization and early development are regulated at the level of translation, post- translational modification and proteolysis, and not by transcription. Proteomics is the only tool for systematically investigating these levels of regulation and discovering new mechanisms. We propose to develop a cutting edge proteomic research platform for Xenopus embryos and egg extract by migrating technology developed in human and yeast systems, and to publicly disseminate the databases and tools we develop. We will also optimize methods for proteomic analysis of protein complexes and compartments in egg extract. Using these tools we will quantify relative amounts of >5,000 proteins, >10,000 phosphorylation sites and >1,500 ubiquitination sites in early development and in egg extracts under normal and perturbed conditions. We will investigate the mechanism of cell cycle regulation, how the cell cycle changes during early development, how microtubules are nucleated, and how embryos detect changes in the nucleus to cytoplasm ratio.

Public Health Relevance

We will develop broad based tools for the study of changes in the protein levels during the cell division cycle, bringing the revolutionary technique of mass spectrometry to embryonic systems. Such studies could offer a better basis for designing drugs that affect important pathways in cancer and inflammation.

National Institute of Health (NIH)
Research Project (R01)
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Special Emphasis Panel (ZRG1)
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Hamlet, Michelle R
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Harvard Medical School
Schools of Medicine
United States
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Nguyen, Phuong A; Groen, Aaron C; Loose, Martin et al. (2014) Spatial organization of cytokinesis signaling reconstituted in a cell-free system. Science 346:244-7
Gujral, Taranjit S; Chan, Marina; Peshkin, Leonid et al. (2014) A noncanonical Frizzled2 pathway regulates epithelial-mesenchymal transition and metastasis. Cell 159:844-56
Gujral, Taranjit Singh; Peshkin, Leonid; Kirschner, Marc W (2014) Exploiting polypharmacology for drug target deconvolution. Proc Natl Acad Sci U S A 111:5048-53
McAlister, Graeme C; Nusinow, David P; Jedrychowski, Mark P et al. (2014) MultiNotch MS3 enables accurate, sensitive, and multiplexed detection of differential expression across cancer cell line proteomes. Anal Chem 86:7150-8
Wühr, Martin; Freeman Jr, Robert M; Presler, Marc et al. (2014) Deep proteomics of the Xenopus laevis egg using an mRNA-derived reference database. Curr Biol 24:1467-75