Retrograde signaling is a communication pathway from organelles to nucleus central to regulation and coordination of numerous processes in living organisms, including developmental progressions, responses to biotic and abiotic challenges, protein trafficking and remodeling of chromatin structure. Recently, we have identified the first stress-specific retrograde plastid-to-nucleus signaling metabolite, methylerythritol cyclodiphosphate (MEcPP), an isoprenoid precursor produced by the methylerythritol phosphate (MEP) pathway. This is a conserved signaling pathway that is present in plants, pathogenic bacteria, and in the non- photosynthetic plastids """"""""apicoplast"""""""" of the malarial parasite, but is absent in animals. The long-term goal of our research is to gain insight into the regulatory components involved in perception and transduction of the MEcPP signaling cascade that underlies plant responses to stress. Towards this goal, in a collaborative study, we propose to target the following specific objectives: 1: Define cellular components of the MEcPP signaling pathway using genetic and biochemical approaches. 2: Define epigenetic regulators modulated during MEcPP signaling pathway by studying MEcPP regulated class II histone deacetylase (HDAC), and by global mapping of MEcPP-mediated chromatin structural modifications. 3: Determine the role of plastid stromules (stroma filled tubules) in MEcPP-mediated plastid-to-nucleus retrograde signaling using proteomics and cell biological approaches. To undertake these specific objectives we have generated foundational data and unmatched experimental tools including suppressor lines reverted in their ability to induce stress response genes in spite of high MEcPP levels, microarray-based identification of histone deacetylase gene as potential epigenetic regulators of stress responses, and plastid expressing fluorescent protein lines that enable microscopy-based identification of chloroplast tubular extensions called """"""""stromules"""""""" found in plants with enhanced MEcPP levels.

Public Health Relevance

Plastids are biochemically intensive organelles that sense environmental and developmental cues and coordinate appropriate responses with the nucleus. We have recently identified a small, plastid-derived metabolite that functions as a stress sensor in both plants and eubacteria. Our proposed experiments will provide fundamental insights into poorly understood signaling mechanisms through which plastids signal perception of stress to the nucleus. The metabolic pathway responsible for production of this signal is an ideal target for development of new drugs since it is present in pathogenic bacteria and the malarial parasite, but is absent in animals. Successful execution of the proposed study will, therefore, not only provide fundamental insight into the mechanism of plastid-to-nucleus signaling, but also offers novel candidates for development of new antibiotics and antimalarial agents.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
1R01GM107311-01
Application #
8559415
Study Section
Cellular Signaling and Regulatory Systems Study Section (CSRS)
Program Officer
Maas, Stefan
Project Start
2013-09-05
Project End
2017-08-31
Budget Start
2013-09-05
Budget End
2014-08-31
Support Year
1
Fiscal Year
2013
Total Cost
$279,044
Indirect Cost
$89,044
Name
University of California Davis
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
047120084
City
Davis
State
CA
Country
United States
Zip Code
95618
Lemos, Mark; Xiao, Yanmei; Bjornson, Marta et al. (2016) The plastidial retrograde signal methyl erythritol cyclopyrophosphate is a regulator of salicylic acid and jasmonic acid crosstalk. J Exp Bot 67:1557-66
Bjornson, Marta; Dandekar, Abhaya; Dehesh, Katayoon (2016) Determinants of timing and amplitude in the plant general stress response. J Integr Plant Biol 58:119-26
Benn, Geoffrey; Bjornson, Marta; Ke, Haiyan et al. (2016) Plastidial metabolite MEcPP induces a transcriptionally centered stress-response hub via the transcription factor CAMTA3. Proc Natl Acad Sci U S A 113:8855-60
Taylor-Teeples, M; Lin, L; de Lucas, M et al. (2015) An Arabidopsis gene regulatory network for secondary cell wall synthesis. Nature 517:571-5
Walley, Justin; Xiao, Yanmei; Wang, Jin-Zheng et al. (2015) Plastid-produced interorgannellar stress signal MEcPP potentiates induction of the unfolded protein response in endoplasmic reticulum. Proc Natl Acad Sci U S A 112:6212-7
Wang, Changquan; Dehesh, Katayoon (2015) From retrograde signaling to flowering time. Plant Signal Behav 10:e1022012
Wang, Chang-Quan; Sarmast, Mostafa Khoshhal; Jiang, Jishan et al. (2015) The Transcriptional Regulator BBX19 Promotes Hypocotyl Growth by Facilitating COP1-Mediated EARLY FLOWERING3 Degradation in Arabidopsis. Plant Cell 27:1128-39
Bjornson, Marta; Song, Xingshun; Dandekar, Abhaya et al. (2015) A Chemical Genetic Screening Procedure for Arabidopsis thaliana Seedlings. Bio Protoc 5:
Savchenko, Tatyana; Dehesh, Katayoon (2014) Drought stress modulates oxylipin signature by eliciting 12-OPDA as a potent regulator of stomatal aperture. Plant Signal Behav 9:e28304
Grimm, Bernhard; Dehesh, Kayatoon; Zhang, Lixin et al. (2014) Intracellular communication. Mol Plant 7:1071-4

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