Cell-cell adhesion defines solid tissues, and dysregulation of adhesion is an essential step in cancer cell metastasis. The protein aE-catenin has critical roles in cell and tissue development by transducing mechanical tension between cadherin cell adhesion molecules and the actin cytoskeleton into biochemical signals. We will investigate the molecular basis of how aE-catenin structure changes in response to force, and how its molecular behavior contributes to the formation and dissociation of cell-cell contacts. Our approach is to use a combination of rigorous biochemical characterization (Weis) and innovative single-molecule optical trapping assays (Dunn) to discover how the protein a-catenin both reinforces cell-cell junctions and triggers downstream signal transduction in response to mechanical stress. This question has deep biomedical significance, since a-catenin is known to be required for the formation of multicellular tissues and is a central player in both organogenesis and cancer metastasis. Cell biological data show that a-catenin and its binding partner -catenin are required to link the intracellular adhesion protein E-cadherin (epithelial cadherin) to the actin cytoskeleton. However, the a- catenin/-catenin/E-cadherin does not bind actin in bulk biochemical assays. In preliminary work, we used a novel single-molecule optical trap assay to show that the cadherin/catenin ternary complex can indeed bind actin, but only in the presence of mechanical load. Further, we find that the strength of the a-catenin-actin bond increases with mechanical load, and that binding of the cadherin/catenin complex to the actin filament is highly cooperative. The implication of these findings is that a-catenin acts as a force sensitive linker that can reinforce cell-cell contacts in response to mechanical load. This mechanism provides an elegant means to maintain tissue integrity in the presence of mechanical strain, and provides an explanation for how cells may sense tension at cell-cell junctions, a topic of intense current interest. However, how exactly a-catenin senses mechanical tension is not known. We will use a combination of biochemical and single-molecule biophysical approaches to: 1) determine the molecular mechanism by which a-catenin forms a force-sensitive linkage between cadherins and the actin cytoskeleton; and 2) discover how cooperative structural transformations in a-catenin, actin, or both regulate binding between the cadherin/catenin complex and filamentous actin. These measurements will reveal the molecular mechanism by which a-catenin senses force at cell-cell junctions. In addition, this work will provide a mechanistic basis for understanding how groups of cadherin-catenin complexes work in concert to remodel cell-cell junctions in response to changes in mechanical load, with potentially broad implications for our understanding of epithelial remodeling and morphogenesis.

Public Health Relevance

The cells of solid tissues and organs adhere to one another at specific cell-cell junctions that can respond to mechanical force to maintain tissue integrity and to remodel tissues during development. The goal of this proposal is to understand how a key protein in these junctions responds to force, and how its molecular behavior contributes to the formation and disassembly of cell-cell contacts. This study will significantly advance our understanding of the role of cell-cell junctions in normal tissue development and maintenance as well as in cancer, in which loss of cell-cell contacts is a key step during metastasis.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
1R01GM114462-01A1
Application #
8964322
Study Section
Macromolecular Structure and Function C Study Section (MSFC)
Program Officer
Nie, Zhongzhen
Project Start
2015-09-22
Project End
2019-04-30
Budget Start
2015-09-22
Budget End
2016-04-30
Support Year
1
Fiscal Year
2015
Total Cost
Indirect Cost
Name
Stanford University
Department
Engineering (All Types)
Type
Biomed Engr/Col Engr/Engr Sta
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94304
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Kang, Hyunook; Bang, Injin; Jin, Kyeong Sik et al. (2017) Structural and functional characterization of Caenorhabditis elegans ?-catenin reveals constitutive binding to ?-catenin and F-actin. J Biol Chem 292:7077-7086
Shao, Xiangqiang; Kang, Hyunook; Loveless, Timothy et al. (2017) Cell-cell adhesion in metazoans relies on evolutionarily conserved features of the ?-cateninĀ·?-catenin-binding interface. J Biol Chem 292:16477-16490