Electrical signaling in bacterial biofilms Grol Sel (P.I.), Lev Tsimring (Co-I.) and Andrew Mugler (Co-I.) Summary Understanding communication among bacteria is a fundamental biological problem with critical implications for public health. Nowhere is this problem perhaps more relevant than in bacterial communities known as biofilms. How individual bacteria communicate within such collectively organized communities to coordinate their behavior is a long-standing question in biology. We recently discovered a new type of bacterial communication mechanism based on ion channel mediated electrical cell-to-cell signaling in Bacillus subtilis biofilms. This discovery made possible by our recent technical innovations, ideally positions us to tackle the fundamental and long-standing question regarding how bacteria coordinate their behavior in biofilms. Necessitated by the multi- scale nature of the problem, the specific aims we propose are designed to bridge across spatial and temporal scales: 1) How does cell density and heterogeneity influence long-range communication within the biofilm? We propose that high cell density and cell-to-cell heterogeneity in electrical activity promotes efficient signal transmission within the biofilm. 2) What governs the composition and dispersion of biofilm communities? We propose that long-range electrical signaling plays a critical role in attracting individual bacteria to, or repelling them from the biofilm. 3) Does a biofilm operate as an independent functional unit in the presence of neighboring biofilms? We propose that multiple neighboring biofilms can become coupled through long-range electrical signaling into a super-cluster that can act as a functional unit. These questions involve phenomena operating over length scales ranging from individual cells to groups of biofilms, and timescales ranging from seconds to hours. Therefore, multi-scale quantitative time-lapse microscopy is the essential tool to address these questions. Importantly, the resulting quantitative spatio-temporal measurements are ideal to inform and constrain problem specific mathematical models and determine the role of electrical signaling in coordinating bacteria in biofilms. The quantitative spatio-temporal measurements to be generated here will thus also serve the broad interests of the computational biology community. !

Public Health Relevance

to Public Health: Bacteria naturally form communities known as biofilms, which are responsible for two thirds of all clinical infections. Biofilms pose a serious threat to public health due to their 1000 fold higher resistance against antibiotics compared to individual bacteria. Furthermore, biofilms are the major sites of bacterial spore formation that can be highly toxic to humans. Motivated by our recent discovery and preliminary results, we propose to explore how bacteria coordinate their behavior in biofilm communities by exploring the role of electrical cell-to-cell signaling. Uncovering the fundamental principles that govern coordination and collective dynamics of bacteria within biofilms will identify new approaches to control biofilm formation and dispersion providing us with potentially new tools to combat this public health threat.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM121888-02
Application #
9394021
Study Section
Modeling and Analysis of Biological Systems Study Section (MABS)
Program Officer
Brazhnik, Paul
Project Start
2016-12-09
Project End
2020-11-30
Budget Start
2017-12-01
Budget End
2018-11-30
Support Year
2
Fiscal Year
2018
Total Cost
Indirect Cost
Name
University of California San Diego
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
804355790
City
La Jolla
State
CA
Country
United States
Zip Code
92093
Humphries, Jacqueline; Xiong, Liyang; Liu, Jintao et al. (2017) Species-Independent Attraction to Biofilms through Electrical Signaling. Cell 168:200-209.e12
Liu, Jintao; Martinez-Corral, Rosa; Prindle, Arthur et al. (2017) Coupling between distant biofilms and emergence of nutrient time-sharing. Science 356:638-642