Abstract

Pregnant mare serum gonadotropin (PMSG) is a unique glycoprotein hormone with the biological activities of both the pituitary gonadotropins, follicle stimulating hormone (FSH) and luteinizing hormone (LH). PMSG is also the only gonadotropin which can presently be isolated from the blood in pure form. The proposed studies on PMSG are designed to gain information both on a hormone closely related to FSH and LH but also on the nature of a hormone as it appears in the circulation. Techniques of protein-polypeptide chemistry are employed to obtain highly purified PMSG and subunits for biochemical, biological, and immunological studies. In vivo and in vitro bioassays for FSH and LH are used to obtain structure-function correlates. In these studies, PMSG and its subunits are studied chemically and compared to pituitary FSH, LH and their subunits. The hormone from the endometrial cups and urinary excretion products are also studied. These studies are intended to give greater knowledge of the mechanism of gonadotropin action and reproductive function on the molecular level.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD005722-13
Application #
3310407
Study Section
Biochemical Endocrinology Study Section (BCE)
Project Start
1978-07-01
Project End
1986-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
13
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Type
Schools of Medicine
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

McFarlane, J R; Czekala, N M; Papkoff, H (1991) Zebra chorionic gonadotropin: partial purification and characterization. Biol Reprod 44:827-33
McFarlane, J R; Coulson, S A; Papkoff, H (1991) Biological and immunoactive substances resembling chorionic gonadotropin are present in full-term horse and zebra placentas. Biol Reprod 45:343-9
McFarlane, J R; Cabrera, C M; Coulson, S A et al. (1991) Partial purification and characterization of rhinoceros gonadotropins, growth hormone, and prolactin: comparison with the horse and sheep. Biol Reprod 44:94-101
Tortonese, D J; Lewis, P E; Papkoff, H et al. (1990) Roles of the dominant follicle and the pattern of oestradiol in induction of preovulatory surges of LH and FSH in prepubertal heifers by pulsatile low doses of LH. J Reprod Fertil 90:127-35
McFarlane, J R; Cabrera, C M; Oosthuizen, M M et al. (1990) Elephant pituitary gonadotropins. Gen Comp Endocrinol 79:193-200
Bergert, E R; Madden, B; McCormick, D J et al. (1990) The antigenic structure of the human glycoprotein hormone alpha-subunit: II. Cross-species comparisons. Endocrinology 127:2985-9
Matteri, R L; Papkoff, H (1988) Microheterogeneity of equine follicle-stimulating hormone. Biol Reprod 38:324-31
Matteri, R L; Papkoff, H (1987) Characterization of equine luteinizing hormone by chromatofocusing. Biol Reprod 36:261-9
Matteri, R L; Baldwin, D M; Lasley, B L et al. (1987) Biological and immunological properties of zebra pituitary gonadotropins: comparison with horse and donkey gonadotropins. Biol Reprod 36:1134-41
Matteri, R L; Roser, J F; Baldwin, D M et al. (1987) Characterization of a monoclonal antibody which detects luteinizing hormone from diverse mammalian species. Domest Anim Endocrinol 4:157-65

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