Oxygen deprivation, typically the result of a combination of hypoxia and hypoperfusion, is often the primary event setting off a biochemical cascade that leads to irreversible brain injury in perinatal encephalopathy. The long-term objective of this project is the development of a novel near-infrared spectroscopy (NIRS) device for the continuous monitoring of the regional tissue saturation (St02), arterial saturation (Sa02), and venous saturation (Sv02) in the neonate's brain. Such a device could potentially detect brain tissue compromise before the development of irreversible structural damage, thus allowing for timely and specific intervention. The novelty of our approach relates to the hypothesis that an appropriate temporal analysis of NIRS data can yield three different oxygenation measurements: (1) St02 from the temporal average of the optical signal; (2) Sa02 from the oscillating component of the optical absorption at the pulse frequency; (3) Sv02 from the oscillating component of the optical absorption at the respiratory frequency. The short-term goals of this project are to characterize NIRS measurements of cerebral oxygenation and to identify the most effective practical implementation of NIRS (in terms of instrumentation and data processing) for non-invasive cerebral oximetry. In particular, we aim to 1 ) investigate the relationship between the local measurements of the hemoglobin oxygenation in the cerebral tissue, in the arterial vascular compartment, and in the venous vascular compartment, and 2) perform spatially resolved measurements of cerebral oxygenation to localize areas of brain damage. To achieve our short-term goals, we propose to perform measurements on an animal model (piglets) where we can induce changes in the hemoglobin oxygenation levels over a large range, where we can perform hemodilution experiments, where we can induce focal brain injury, and where we have access to invasive readings of Sa02 and Sv02 from the gas analysis of blood samples. Finally we will test our proposed NIRS approach to cerebral oximetry on human neonates. The completion of this study will lead to the characterization of NIRS oximetry of the brain, to the development of multimode optical oximetry (simultaneous local measurement of St02, Sa02, and Sv02), and to the identification of the most effective approach to the non-invasive. continuous monitoring of cerebral oxygenation in neonates.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD042908-03
Application #
6729110
Study Section
Diagnostic Imaging Study Section (DMG)
Program Officer
Vitkovic, Ljubisa
Project Start
2002-07-01
Project End
2006-04-30
Budget Start
2004-05-01
Budget End
2005-04-30
Support Year
3
Fiscal Year
2004
Total Cost
$372,470
Indirect Cost
Name
Massachusetts General Hospital
Department
Type
DUNS #
073130411
City
Boston
State
MA
Country
United States
Zip Code
02199
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Dehaes, Mathieu; Cheng, Henry H; Buckley, Erin M et al. (2015) Perioperative cerebral hemodynamics and oxygen metabolism in neonates with single-ventricle physiology. Biomed Opt Express 6:4749-67
Buckley, Erin M; Parthasarathy, Ashwin B; Grant, P Ellen et al. (2014) Diffuse correlation spectroscopy for measurement of cerebral blood flow: future prospects. Neurophotonics 1:
Roche-Labarbe, Nadege; Fenoglio, Angela; Radhakrishnan, Harsha et al. (2014) Somatosensory evoked changes in cerebral oxygen consumption measured non-invasively in premature neonates. Neuroimage 85 Pt 1:279-86
Dehaes, Mathieu; Aggarwal, Alpna; Lin, Pei-Yi et al. (2014) Cerebral oxygen metabolism in neonatal hypoxic ischemic encephalopathy during and after therapeutic hypothermia. J Cereb Blood Flow Metab 34:87-94
Lin, Pei-Yi; Roche-Labarbe, Nadège; Dehaes, Mathieu et al. (2013) Regional and hemispheric asymmetries of cerebral hemodynamic and oxygen metabolism in newborns. Cereb Cortex 23:339-48
Lin, Pei-Yi; Roche-Labarbe, Nadege; Dehaes, Mathieu et al. (2013) Non-invasive optical measurement of cerebral metabolism and hemodynamics in infants. J Vis Exp :e4379
Roche-Labarbe, Nadège; Fenoglio, Angela; Aggarwal, Alpna et al. (2012) Near-infrared spectroscopy assessment of cerebral oxygen metabolism in the developing premature brain. J Cereb Blood Flow Metab 32:481-8
Zimmermann, B B; Roche-Labarbe, N; Surova, A et al. (2012) The confounding effect of systemic physiology on the hemodynamic response in newborns. Adv Exp Med Biol 737:103-9

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