Bacteriophage Cloning System - High Molecular Weight DNA

Hollis, Gregory F.

Abstract

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Human Genome Research Institute (NHGRI)
• Type: Research Project (R01)
• Project #: 5R01HG000339-08
• Application #: 2208753
• Study Section: Mammalian Genetics Study Section (MGN)
• Project Start: 1989-08-01
• Project End: 1997-02-28
• Budget Start: 1996-03-27
• Budget End: 1997-02-28
• Support Year: 8
• Fiscal Year: 1996

Institution

• Name: Du Pont Merck Pharmaceutical Company
• City: Wilmington
• State: DE
• Country: United States
• Zip Code: 19880

Publications

Coren, J S; Sternberg, N (2001) Construction of a PAC vector system for the propagation of genomic DNA in bacterial and mammalian cells and subsequent generation of nested deletions in individual library members. Gene 264:11-8

Coren, J S; Pierce, J C; Sternberg, N (1995) Headful packaging revisited: the packaging of more than one DNA molecule into a bacteriophage P1 head. J Mol Biol 249:176-84

Skorupski, K; Sternberg, N; Sauer, B (1994) Purification and DNA-binding activity of the PacA subunit of the bacteriophage P1 pacase enzyme. J Mol Biol 243:258-67

Albertsen, H M; Smith, S A; Mazoyer, S et al. (1994) A physical map and candidate genes in the BRCA1 region on chromosome 17q12-21. Nat Genet 7:472-9

Skorupski, K; Sauer, B; Sternberg, N (1994) Faithful cleavage of the P1 packaging site (pac) requires two phage proteins, PacA and PacB, and two Escherichia coli proteins, IHF and HU. J Mol Biol 243:268-82

Pierce, J C; Sternberg, N; Sauer, B (1992) A mouse genomic library in the bacteriophage P1 cloning system: organization and characterization. Mamm Genome 3:550-8

Pierce, J C; Sauer, B; Sternberg, N (1992) A positive selection vector for cloning high molecular weight DNA by the bacteriophage P1 system: improved cloning efficacy. Proc Natl Acad Sci U S A 89:2056-60