Our efforts to develop a physical map of chromosome 3 have resulted in well localized YACs and YAC contigs for approximately 70% of the chromosome, as well as an extensive collection of molecular probes and somatic cell hybrids. These studies provide a powerful foundation for subsequent efforts aimed at improving the fidelity and detail of the physical map, steps essential for achieving the goals of the Genome Project. We propose to build on this foundation by converting the present coarse physical map into one which is highly detailed, integrated, extremely useful and ready for DNA sequence analysis. We propose to complete development of the YAC contig, construct additional contigs from smaller clones of higher fidelity, develop STS markers every 100 kb and a transcriptional map for select chromosome 3 segments, as well as to interface our chromosome 3 database with other systems (such as IGD) in order to improve accessibility and distribution of results. In this proposal, robotics and automation are essential components of the experimental design. This proposal addresses several goals of the Genome Project including the development of a highly detailed physical map for chromosome 3 using clones with improved stability and lowered chimerism, developing of a high density STS map, improvement in gene isolation techniques coupled with generation of a transcriptional map for several multi-megabase regions and continued development of software for storing and searching through mapping information.

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Research Project (R01)
Project #
2R01HG000358-07
Application #
2208772
Study Section
Genome Study Section (GNM)
Project Start
1989-07-01
Project End
1997-06-30
Budget Start
1994-09-01
Budget End
1995-06-30
Support Year
7
Fiscal Year
1994
Total Cost
Indirect Cost
Name
University of Colorado Denver
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
065391526
City
Aurora
State
CO
Country
United States
Zip Code
80045
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Korch, C; Drabkin, H (1999) Manganese citrate improves base-calling accuracy in DNA sequencing reactions using rhodamine-based fluorescent dye-terminators. Nucleic Acids Res 27:1405-7
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van den Berg, A; van der Veen, A Y; Hulsbeek, M M et al. (1995) Defining the position of the breakpoint of the constitutional t(3;6) occurring in a family with renal cell carcinoma. Genes Chromosomes Cancer 12:224-8
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