Abstract

investigator's specific aims) The specific aims of this revised application are: 1) Isolate full length mucin cDNAs from rat and human airway libraries; from these determine the primary structure of mucin core protein in both species. 2) Using the rat mucin cDNA, isolate the cognate gene from a rat genomic library and identify upstream (or other) regulatory elements using transfection analysis of deletion mutants. 3) Determine the extent to which these regulatory elements bind nuclear proteins using mobility shift and dNase I protection assays and 4) Establish a rat model of respiratory mucin overproduction and determine whether transcriptional control of mucin biosynthesis is upregulated using a rat mucin cDNA in northern hybridization and nuclear run-on assays.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL043762-05
Application #
2221166
Study Section
Respiratory and Applied Physiology Study Section (RAP)
Project Start
1990-07-01
Project End
1995-07-31
Budget Start
1994-07-01
Budget End
1995-07-31
Support Year
5
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

Sidhu, Sukhvinder S; Mengistab, Aklilu T; Tauscher, Andrew N et al. (2004) The microvesicle as a vehicle for EMMPRIN in tumor-stromal interactions. Oncogene 23:956-63
Gensch, Erin; Gallup, Marianne; Sucher, Anatol et al. (2004) Tobacco smoke control of mucin production in lung cells requires oxygen radicals AP-1 and JNK. J Biol Chem 279:39085-93
McNamara, Nancy; Gallup, Marianne; Khong, Amy et al. (2004) Adenosine up-regulation of the mucin gene, MUC2, in asthma. FASEB J 18:1770-2
Lemjabbar, Hassan; Li, Daizong; Gallup, Marianne et al. (2003) Tobacco smoke-induced lung cell proliferation mediated by tumor necrosis factor alpha-converting enzyme and amphiregulin. J Biol Chem 278:26202-7
McNamara, Nancy; Basbaum, Carol (2002) Mechanism by which bacterial flagellin stimulates host mucin production. Adv Exp Med Biol 506:269-73
McNamara, N; Khong, A; McKemy, D et al. (2001) ATP transduces signals from ASGM1, a glycolipid that functions as a bacterial receptor. Proc Natl Acad Sci U S A 98:9086-91
Longphre, M; Li, D; Li, J et al. (2000) Lung mucin production is stimulated by the air pollutant residual oil fly ash. Toxicol Appl Pharmacol 162:86-92
Longphre, M; Li, D; Gallup, M et al. (1999) Allergen-induced IL-9 directly stimulates mucin transcription in respiratory epithelial cells. J Clin Invest 104:1375-82
Nogami, H; Ohmori, H; Li, J D et al. (1997) Sp1 protein contributes to airway-specific rat MUC 2 mucin gene transcription. Gene 198:191-201
Kai, H; Takeuchi, K; Ohmori, H et al. (1996) Transcriptional regulation of the lysozyme gene in airway gland serous cells. J Cell Biochem 61:350-62

Showing the most recent 10 out of 18 publications