The hypothesis of this application is that in vitro expanded yolk sac hematopoietic stem cells can successfully engraft in histoincompatible hosts in which they then differentiate, thereby establishing immune functions.
The specific aims are to: 1) establish conditions that promote self-renewal of yolk sac-derived mouse hematopoietic stem cells in vitro; 2) determine the ability of the expanded yolk sac-derived stem population (a) to seed murine target organs in histocompatible host animals; (b) to generate the various blood cell lineages; and (c) to yield cells capable of functioning in the immune system; and 3) determine whether the expanded yolk sac-derived stem cell population can also seed murine target organs and differentiate in histoincompatible host animals.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL059370-02
Application #
2857944
Study Section
Human Embryology and Development Subcommittee 1 (HED)
Project Start
1998-01-01
Project End
2000-12-31
Budget Start
1999-01-01
Budget End
1999-12-31
Support Year
2
Fiscal Year
1999
Total Cost
Indirect Cost
Name
University of Wisconsin Madison
Department
Other Health Professions
Type
Schools of Medicine
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
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Plendl, Johanna; Gilligan, Barbara J; Wang, Shur-Jen et al. (2002) Primitive endothelial cell lines from the porcine embryonic yolk sac. In Vitro Cell Dev Biol Anim 38:334-42
Alferiev, Ivan S; Fishbein, Ilia (2002) Activated polyurethane modified with latent thiol groups. Biomaterials 23:4753-8