Post-translational modifications of endothelial nitric oxide synthase (eNOS) play a very important part in regulating eNOS activity, endothelial nitric oxide (NO) production, endothelium-dependent vasorelaxation, and vascular tone. The roles of phosphorylation, acylation, and glycosylation in regulating eNOS activity are well recognized. However, the importance of acetylation, as a post-translational modification, in governing eNOS activity has not been carefully studied. Based on novel preliminary evidence, this application proposes that eNOS is acetylated in the endothelium, and site-specific de-acetylation of eNOS has an important role in governing eNOS activity, endothelium-dependent vasorelaxation, and vascular tone. We suggest that the ubiquitously expressed protein deacetylase SIRT1 (Silent Information RegulaTor), by de-acetylating eNOS at specific acetylated lysine residues, stimulates eNOS activity, endothelial NO production, and promotes endothelium-dependent vasorelaxation. This application will examine if eNOS is a direct target of SIRT1, and characterize in detail the mechanism through which SIRT1 stimulates eNOS activity. The importance of a SIRT1-dependent mechanism in governing endothelium-dependent vascular relaxation, and the mediating role of eNOS in this mechanism, will be assessed. The in vivo relevance of SIRT1 with respect to modulation of physiologic changes in vascular tone will be determined. Finally, the regulation of human SIRT1 expression by a redox-sensitive transcriptional mechanism involving the p53 transcription factor and its transcriptional co-activator redox factor-1, and the physiologic importance of this transcriptional mechanism in regulating vascular SIRT1 expression, will be explored. In summary, this application will determine if eNOS is a target of the SIRT1 deacetylase, and in doing so, promises to define a novel mechanism for the regulation of endothelium-dependent vascular relaxation. It will also explore the physiologic importance of this mechanism in controlling vascular tone in vivo. As such, it may offer new targets and strategies for the treatment of human disorders of endothelial function and dysregulation of vascular tone.

Public Health Relevance

Production of nitric oxide in blood vessels is a very important means for the regulation of blood vessel function. This project examines a novel means by which production of nitric oxide in blood vessels is controlled. Understanding this new mechanism that controls nitric oxide in blood vessels will help further our knowledge about how blood vessels in disease states such as high blood pressure and atherosclerosis have lower levels of nitric oxide.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL070929-09
Application #
7806369
Study Section
Vascular Cell and Molecular Biology Study Section (VCMB)
Program Officer
Gao, Yunling
Project Start
2002-08-01
Project End
2012-03-31
Budget Start
2010-04-01
Budget End
2011-03-31
Support Year
9
Fiscal Year
2010
Total Cost
$340,875
Indirect Cost
Name
University of Pittsburgh
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
004514360
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213
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Irani, Kaikobad (2011) Crippling of Kr├╝ppel (-like factor 2) by bad flow portends a miRky day for endothelial function. Circulation 124:541-3
Kumar, Ajay; Kim, Cuk-Seong; Hoffman, Timothy A et al. (2011) p53 impairs endothelial function by transcriptionally repressing Kruppel-Like Factor 2. Arterioscler Thromb Vasc Biol 31:133-41
Kim, Cuk-Seong; Kim, Young-Rae; Naqvi, Asma et al. (2011) Homocysteine promotes human endothelial cell dysfunction via site-specific epigenetic regulation of p66shc. Cardiovasc Res 92:466-75
Yamamori, Tohru; DeRicco, Jeremy; Naqvi, Asma et al. (2010) SIRT1 deacetylates APE1 and regulates cellular base excision repair. Nucleic Acids Res 38:832-45
Naqvi, Asma; Hoffman, Timothy A; DeRicco, Jeremy et al. (2010) A single-nucleotide variation in a p53-binding site affects nutrient-sensitive human SIRT1 expression. Hum Mol Genet 19:4123-33
Jung, Saet-Byel; Kim, Cuk-Seong; Naqvi, Asma et al. (2010) Histone deacetylase 3 antagonizes aspirin-stimulated endothelial nitric oxide production by reversing aspirin-induced lysine acetylation of endothelial nitric oxide synthase. Circ Res 107:877-87
Kumar, Ajay; Hoffman, Timothy A; Dericco, Jeremy et al. (2009) Transcriptional repression of Kruppel like factor-2 by the adaptor protein p66shc. FASEB J 23:4344-52
Santhanam, Lakshmi; Lim, Hyun Kyo; Lim, Hyun Kyoung et al. (2007) Inducible NO synthase dependent S-nitrosylation and activation of arginase1 contribute to age-related endothelial dysfunction. Circ Res 101:692-702

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