(Pro)renin receptor (PRR) is a newly discovered component of the renin-angiotensin system. The exact physiologic and pathologic functions of PRR are not established yet. Our preliminary studies in vitro and in vivo demonstrated that hyperglycemia increases PRR expression and activity, a process that is regulated by angiotensin AT1 receptor (AT1R), mainly via their heterodimerization. As a result of the interaction between AT1R and PRR there is upregulation of V-ATPase and Wnt3a expression and activity leading to renal inflammation fibrosis and albuminuria. In vivo intrarenal interstitia administration of PRR shRNA or Wnt3a shRNA reduced albuminuria, renal inflammation and morphologic changes associated with hyperglycemia. Based on these data, it is likely that PRR directly contributes to development of hyperglycemia-induced renal injury. The long-term goal of our research program is to elucidate the in vitro and in vivo novel mechanisms contributing to the regulation of PRR expression and function and evaluate the pathological significance of this receptor interaction with AT1R, vacuolar-ATPase (V-ATPase) and Wnt3a, and lead to development of hyperglycemia- induced renal disease. To achieve this goal, we will utilize a rationale and novel integrated approaches, consisting of in vivo studies utilizing combined knockdown of renal PRR, V-ATPase or Wnt3a and renal interstitial microdialysis technique in conscious normoglycemic and hyperglycemic streptozotocin- induced diabetes rats. These studies will be complemented by state-of-the-art in vitro cellular and molecular techniques utilizing siRNA, shRNA, EMSA, ChIP assays, and Laser Scanning Confocal FRET microscopy to rigorously test the proposed hypothesis. Based on our recent observations and preliminary data, the central hypothesis of this proposal is that in presence of hyperglycemia, PRR activity is increased by dimerization with AT1R leading to enhanced expression and activity of V-ATPase and Wnt3a signaling pathways to induce renal inflammation, fibrosis and albuminuria. We will pursue the following specific aims:
Aim 1 : To test the hypothesis that hyperglycemia increases PRR expression and activity and its heterodimerization with AT1R, leading to renal inflammation, fibrosis and albuminuria.
Aim 2 : To test the hypothesis that hyperglycemia- enhanced PRR activity mediates renal injury by increasing the expression and activity of V-ATPase.
Aim 3 : To test the hypothesis that hyperglycemia-enhanced PRR activity contributes to renal injury via increased expression and activity of Wnt3a. These studies are expected to identify novel pathophysiologic mechanisms related to PRR and could lead to development of new therapeutic strategies for treating hyperglycemia-induced renal disease.

Public Health Relevance

Kidney disease is a common and morbid complication of diabetes and the leading cause of chronic kidney disease in the developed world. Approximately 40% of persons with diabetes develop kidney disease. It is associated with markedly increased risks of cardiovascular disease and death and higher health care costs. Diabetes-induced kidney disease accounts for nearly half of all incident cases of end-stage renal disease (ESRD) in the United States. Identifying novel pathophysiologic mechanisms induced by (Pro)renin receptor and its contribution to renal injury could lead to development of new therapeutic strategies for treating hyperglycemia-induced renal disease.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
2R01HL091535-11A1
Application #
8512918
Study Section
Hypertension and Microcirculation Study Section (HM)
Program Officer
OH, Youngsuk
Project Start
2008-01-15
Project End
2017-06-30
Budget Start
2013-08-01
Budget End
2014-06-30
Support Year
11
Fiscal Year
2013
Total Cost
$376,040
Indirect Cost
$138,040
Name
University of Virginia
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
065391526
City
Charlottesville
State
VA
Country
United States
Zip Code
22904
Li, Caixia; Siragy, Helmy M (2014) High glucose induces podocyte injury via enhanced (pro)renin receptor-Wnt-?-catenin-snail signaling pathway. PLoS One 9:e89233
Matavelli, Luis C; Siragy, Helmy M (2013) Reduction of aldosterone production improves renal oxidative stress and fibrosis in diabetic rats. J Cardiovasc Pharmacol 61:17-22
Huang, Jiqian; Ledford, Kelly J; Pitkin, William B et al. (2013) Targeted deletion of murine CEACAM 1 activates PI3K-Akt signaling and contributes to the expression of (Pro)renin receptor via CREB family and NF-*B transcription factors. Hypertension 62:317-23
Matavelli, Luis C; Huang, Jiqian; Siragy, Helmy M (2012) Combined aliskiren and amlodipine reduce albuminuria via reduction in renal inflammation in diabetic rats. J Cardiovasc Pharmacol 59:281-7
Huang, Jiqian; Siragy, Helmy M (2012) Sodium depletion enhances renal expression of (pro)renin receptor via cyclic GMP-protein kinase G signaling pathway. Hypertension 59:317-23
Abadir, Peter M; Walston, Jeremy D; Carey, Robert M et al. (2011) Angiotensin II Type-2 receptors modulate inflammation through signal transducer and activator of transcription proteins 3 phosphorylation and TNFýý production. J Interferon Cytokine Res 31:471-4
Matavelli, Luis C; Huang, Jiqian; Siragy, Helmy M (2011) Angiotensin AT? receptor stimulation inhibits early renal inflammation in renovascular hypertension. Hypertension 57:308-13
Huang, Jiqian; Matavelli, Luis C; Siragy, Helmy M (2011) Renal (pro)renin receptor contributes to development of diabetic kidney disease through transforming growth factor-?1-connective tissue growth factor signalling cascade. Clin Exp Pharmacol Physiol 38:215-21
Siragy, Helmy M (2010) Improving vascular function in hypertension: potential benefits of combination therapy with amlodipine and renin-angiotensin-aldosterone system blockers. J Hypertens 28:2-8
Matavelli, Luis C; Huang, Jiqian; Siragy, Helmy M (2010) (Pro)renin receptor contributes to diabetic nephropathy by enhancing renal inflammation. Clin Exp Pharmacol Physiol 37:277-82

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