Most cases of COPD and lung cancer are caused by cigarette smoking. Both diseases start in the airway epithelium, and COPD increases lung cancer risk 4.5-fold. Using airway epithelium genome-wide transcriptome analysis, we and others have concluded that smoking causes a field of molecular changes throughout the airway epithelium manifested by abnormal expression of genes/pathways relevant to COPD and lung cancer. In a paradigm shift from the strategy of assessing the entire differentiated airway epithelium to understand the disordered airway epithelial biology of COPD and lung cancer, this proposal focuses on airway basal cells (BC), the stem/progenitor cells from which the differentiated airway epithelium is derived. Our preliminary observations show that the BC transcriptome of smokers is markedly different from that of nonsmokers, it is a primitive, less differentiated transcriptome including human embryonic stem cell genes progressively up-regulated in COPD and lung cancer, it predicts lung cancer aggressiveness and survival, and is associated with a molecular phenotype of p53 inactivation. Therefore we hypothesize that smoking-dependent reprogramming of the BC transcriptome represents a common molecular pathogenetic mechanism of smoking-associated COPD and lung cancer, thus both "BC disorders," and that the BC transcriptome progressively adopts a cancer-like phenotype in smokers, in smokers with COPD and in the non-malignant airway epithelium of smokers with COPD with lung cancer. If correct, the consequence is a progressive inability of such reprogrammed BC to differentiate to a normal ciliated mucociliary epithelium. These studies should lead to identification of molecular pathways commonly altered in COPD and lung cancer vulnerable to therapeutic intervention to prevent or reverse the disordered BC transcriptome associated with these diseases.
Aim 1 - using genome-wide gene expression profiling, assess the hypothesis that compared to healthy nonsmokers, the airway BC transcriptome of smokers, smokers with COPD and non-malignant epithelium of COPD smokers with lung cancer is progressively disordered and reprogrammed toward a common COPD and cancer-associated molecular phenotype.
Aim 2 - examine the hypothesis that BC of smokers, smokers with COPD and non-malignant epithelium of COPD smokers with lung cancer progressively exhibit a defective capacity to differentiate into normal airway epithelium when placed on air-liquid interface culture but will generate progressively increasing numbers of colonies in clonogenic assays.
Aim 3 - assess the hypothesis that by genetic/pharmacologic modulation of the abnormally expressed genes/pathways in the BC of smokers, COPD smokers and COPD smokers with lung cancer, will reverse their defective differentiation and self-renewal functions.

Public Health Relevance

COPD and lung cancer are the most common smoking-related diseases, which are also pathogenetically linked since COPD conveys a 4.5-fold increased risk for lung cancer. In this study, the common molecular and cellular origins of COPD and lung cancer will be investigated by studying air- way epithelium basal cells, the stem/progenitor cells that renew the airway epithelium. We hypothesize that transcriptional reprogramming of the airway basal cells, which arises prior to histological or clinical disease manifestation, is the central biologic link between COPD and lung cancer.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Project (R01)
Project #
Application #
Study Section
Special Emphasis Panel (ZHL1-CSR-W (F1))
Program Officer
Punturieri, Antonello
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Weill Medical College of Cornell University
Schools of Medicine
New York
United States
Zip Code
Hessel, Justina; Heldrich, Jonna; Fuller, Jennifer et al. (2014) Intraflagellar transport gene expression associated with short cilia in smoking and COPD. PLoS One 9:e85453
Ryan, Dorothy M; Vincent, Thomas L; Salit, Jacqueline et al. (2014) Smoking dysregulates the human airway basal cell transcriptome at COPD risk locus 19q13.2. PLoS One 9:e88051
Staudt, Michelle R; Buro-Auriemma, Lauren J; Walters, Matthew S et al. (2014) Airway Basal stem/progenitor cells have diminished capacity to regenerate airway epithelium in chronic obstructive pulmonary disease. Am J Respir Crit Care Med 190:955-8
Buro-Auriemma, Lauren J; Salit, Jacqueline; Hackett, Neil R et al. (2013) Cigarette smoking induces small airway epithelial epigenetic changes with corresponding modulation of gene expression. Hum Mol Genet 22:4726-38
Walters, Matthew S; Gomi, Kazunori; Ashbridge, Beth et al. (2013) Generation of a human airway epithelium derived basal cell line with multipotent differentiation capacity. Respir Res 14:135
Shaykhiev, Renat; Wang, Rui; Zwick, Rachel K et al. (2013) Airway basal cells of healthy smokers express an embryonic stem cell signature relevant to lung cancer. Stem Cells 31:1992-2002
Shaykhiev, Renat; Zuo, Wu-Lin; Chao, Ionwa et al. (2013) EGF shifts human airway basal cell fate toward a smoking-associated airway epithelial phenotype. Proc Natl Acad Sci U S A 110:12102-7
Shaykhiev, Renat; Crystal, Ronald G (2013) Innate immunity and chronic obstructive pulmonary disease: a mini-review. Gerontology 59:481-9
Shaykhiev, Renat; Sackrowitz, Rachel; Fukui, Tomoya et al. (2013) Smoking-induced CXCL14 expression in the human airway epithelium links chronic obstructive pulmonary disease to lung cancer. Am J Respir Cell Mol Biol 49:418-25
Curradi, Giacomo; Walters, Matthew S; Ding, Bi-Sen et al. (2012) Airway basal cell vascular endothelial growth factor-mediated cross-talk regulates endothelial cell-dependent growth support of human airway basal cells. Cell Mol Life Sci 69:2217-31