Congenital cardiovascular malformations are the most common birth defect and contribute to the morbidity and mortality of our cardiovascular patients. The long range goal and the clinical significance of this proposal are to decipher the networks that govern the progenitors during endothelial development and regeneration. Our laboratory discovered Etv2/Etsrp71/ER71 as a transcriptional target of Nkx2-5 and demonstrated that Etv2 regulates the differentiation of endocardial/endothelial lineages. Additional studies further defined that Etv2 mutant embryos were nonviable and had perturbed mesodermal (endothelial, hematopoietic and cardiac) lineage development. Based on our novel results, our overall hypothesis is that Etv2 is an essential factor for the master molecular program for the endothelial lineage during development and regeneration. In these proposed studies, we will utilize a number of novel genetic models that we have engineered and take an innovative approach to dissect the role of Etv2 as a regulator of endothelial progenitors. To examine our hypotheses, we will address the following specific aims:
Specific Aim #1 : To define the regulation of the Etv2 gene during embryogenesis;
Specific Aim #2 : To define the functional role of microRNAs for the specification and differentiation of the endothelial lineage and Specific Aim #3: To define the cell fate of the Etv2 expressing cells during development and regeneration.
These aims will utilize our recently engineered genetic mouse models and ES cell lines to comprehensively define the role for Etv2 as an essential endothelial regulator and will serve as prelude for therapeutic initiatives to engineer and regenerate the vasculature. Given the tremendous morbidity and mortality of cardiovascular disease in our society, the potential impact of this proposal is significant.

Public Health Relevance

Congenital vascular malformations are a common birth defect and contribute to the morbidity and mortality of our cardiovascular patients. The long range goal and the clinical significance of this proposal are to decipher the networks that govern the progenitors during endothelial development and regeneration. The results from these studies will serve as a prelude for therapeutic initiatives aimed at targeting the endothelial lineage during development, regeneration and disease.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL122576-04
Application #
9237305
Study Section
Vascular Cell and Molecular Biology Study Section (VCMB)
Program Officer
Gao, Yunling
Project Start
2014-04-01
Project End
2018-02-28
Budget Start
2017-03-01
Budget End
2018-02-28
Support Year
4
Fiscal Year
2017
Total Cost
$380,000
Indirect Cost
$130,000
Name
University of Minnesota Twin Cities
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
555917996
City
Minneapolis
State
MN
Country
United States
Zip Code
55455
Gong, Wuming; Kwak, Il-Youp; Koyano-Nakagawa, Naoko et al. (2018) TCM visualizes trajectories and cell populations from single cell data. Nat Commun 9:2749
Gong, Wuming; Kwak, Il-Youp; Pota, Pruthvi et al. (2018) DrImpute: imputing dropout events in single cell RNA sequencing data. BMC Bioinformatics 19:220
Tahara, Naoyuki; Akiyama, Ryutaro; Theisen, Joshua W M et al. (2018) Gata6 restricts Isl1 to the posterior of nascent hindlimb buds through Isl1 cis-regulatory modules. Dev Biol 434:74-83
Koyano-Nakagawa, Naoko; Garry, Daniel J (2017) Etv2 as an essential regulator of mesodermal lineage development. Cardiovasc Res 113:1294-1306
Chen, Zhongming; Zhu, Wuqiang; Bender, Ingrid et al. (2017) Pathologic Stimulus Determines Lineage Commitment of Cardiac C-kit+ Cells. Circulation 136:2359-2372
Gong, Wuming; Rasmussen, Tara L; Singh, Bhairab N et al. (2017) Dpath software reveals hierarchical haemato-endothelial lineages of Etv2 progenitors based on single-cell transcriptome analysis. Nat Commun 8:14362
Garry, Daniel J (2016) Etv2 IS A MASTER REGULATOR OF HEMATOENDOTHELIAL LINEAGES. Trans Am Clin Climatol Assoc 127:212-223
Garry, Glynnis A; Antony, Marie Lue; Garry, Daniel J (2016) Cardiotoxin Induced Injury and Skeletal Muscle Regeneration. Methods Mol Biol 1460:61-71
Garry, Mary G; Garry, Daniel J (2015) Go to the Mattresses: A New Method for Human-Induced Pluripotent Stem Cell-Derived Cardiomyocyte Maturation. Circ Res 117:982-3
Doyle, Michelle J; Lohr, Jamie L; Chapman, Christopher S et al. (2015) Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes as a Model for Heart Development and Congenital Heart Disease. Stem Cell Rev 11:710-27

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