The critical role of the intrauterine environment on neurodevelopment including mental disease risk is becoming increasingly clear, although the mechanisms through which this environment contributes to psychopathology is certainly complex and not well understood. Our work is beginning to demonstrate the fundamental functional role that the placenta, acting as a master regulator of the intrauterine environment and sharing integrated endocrine control with the brain, can play on neurodevelopment. This preliminary work has compelled this examination, which, in line with the strategic mission of the NIMH, aims to provide critical insights into the mechanisms through which the earliest of life's experiences in the intrauterine environment can, through modification of the epigenome, alter neurodevelopmental pathways and contribute to later mental health disorders. The proposed study is compelled by the hypothesis that the pattern of DNA methylation in the placenta acts to alter the function of the placenta as regulator of the intrauterine environment and thus will be associated with infant neurodevelopment in ways that relate to the later development of psychopathology. We propose a multi-modal, translational research approach, utilizing the population resources of an existing, ongoing population-based birth cohort, the validated, prospective, functional infant neurobehavioral assessments using the NICU Network Neurobehavioral Scale (NNNS), and state-of-the-art approaches for the examination of epigenetic and epigenomic profiles in human samples.
The aims of this project are (1) to identify the association between DNA methylation of candidate genes in the placenta and neurobehavioral outcomes at birth using the NNNS (2) to test the hypothesis that profiles of gene-specific DNA methylation of autosomal loci in placenta are associated with neurobehavioral outcomes at birth using the NNNS and to validate these associations, and (3) to test the hypothesis that profiles of gene-specific DNA methylation of sex-linked loci in a sex-stratified manner are associated with neurobehavioral outcomes at birth using the NNNS. The results of this study have the potential to expand our understanding of the developmental origins of mental health, and demonstrate the key role of the placenta and the epigenetic control of its genome in infant neurodevelopment. Identification of an epigenetic signature associated with altered behavioral trajectories in an accessible tissue, such as the placenta, can have significant clinical and public health implications, providing an opportunity for early diagnostic tools which can direct targeted and early interventions for at-risk children.

Public Health Relevance

This research aims to identify the molecular mechanisms through which the effects of the maternal environment elicit lifelong effects on mental health in the newborn child. This work can provide potential avenues for prevention and targeted intervention through the identification of these molecular alterations.

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Research Project (R01)
Project #
5R01MH094609-03
Application #
8499425
Study Section
Special Emphasis Panel (ZMH1-ERB-L (04))
Program Officer
Friedman-Hill, Stacia
Project Start
2011-09-06
Project End
2016-06-30
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
3
Fiscal Year
2013
Total Cost
$466,886
Indirect Cost
$73,556
Name
Dartmouth College
Department
Pharmacology
Type
Schools of Medicine
DUNS #
041027822
City
Hanover
State
NH
Country
United States
Zip Code
03755
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Ciesielski, Timothy H; Aldrich, Melinda C; Marsit, Carmen J et al. (2017) Transdisciplinary approaches enhance the production of translational knowledge. Transl Res 182:123-134
Deyssenroth, Maya A; Li, Qian; LacasaƱa, Marina et al. (2017) Expression of placental regulatory genes is associated with fetal growth. J Perinat Med 45:887-893
Peng, Shouneng; Deyssenroth, Maya A; Di Narzo, Antonio F et al. (2017) Expression quantitative trait loci (eQTLs) in human placentas suggest developmental origins of complex diseases. Hum Mol Genet 26:3432-3441
Sharp, Gemma C; Salas, Lucas A; Monnereau, Claire et al. (2017) Maternal BMI at the start of pregnancy and offspring epigenome-wide DNA methylation: findings from the pregnancy and childhood epigenetics (PACE) consortium. Hum Mol Genet 26:4067-4085
Deyssenroth, Maya A; Peng, Shouneng; Hao, Ke et al. (2017) Whole-transcriptome analysis delineates the human placenta gene network and its associations with fetal growth. BMC Genomics 18:520
Felix, Janine F; Joubert, Bonnie R; Baccarelli, Andrea A et al. (2017) Cohort Profile: Pregnancy And Childhood Epigenetics (PACE) Consortium. Int J Epidemiol :
Johnson, Kevin C; Houseman, E Andres; King, Jessica E et al. (2017) Normal breast tissue DNA methylation differences at regulatory elements are associated with the cancer risk factor age. Breast Cancer Res 19:81
Everson, Todd M; Kappil, Maya; Hao, Ke et al. (2017) Maternal exposure to selenium and cadmium, fetal growth, and placental expression of steroidogenic and apoptotic genes. Environ Res 158:233-244

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