Abstract

The development, function and structure of the neuromuscular junction will be studied. The genes coding for the acetylcholine receptor and the acetylcholine esterase will be cloned using recombinant DNA technology. The structure of these genes will be determined and their regulation studied using probes for mRNA. The expression of the acetylcholine receptor and esterase genes will be measured during development of the neuromuscular junction and after denervation. Mono-clonal antibody specific for antigens of the neuromuscular junction will be produced. These antibodies should be highly specific probes for the study of synapse structure and function. Immunogens will include basement membrane preparations and muscle cells rich in synaptic endings. The elucidation of the defect in the neuromuscular disease, myasthenia gravis, will continue.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS011549-12
Application #
3394493
Study Section
Neurological Sciences Subcommittee 1 (NLS)
Project Start
1977-01-01
Project End
1987-12-31
Budget Start
1985-01-01
Budget End
1985-12-31
Support Year
12
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Salk Institute for Biological Studies
Department
Type
DUNS #
005436803
City
La Jolla
State
CA
Country
United States
Zip Code
92037

  Publications

Vetter, D E; Mann, J R; Wangemann, P et al. (1996) Inner ear defects induced by null mutation of the isk gene. Neuron 17:1251-64
Cauley, K; Marks, M; Gahring, L C et al. (1996) Nicotinic receptor subunits alpha 3, alpha 4, and beta 2 and high affinity nicotine binding sites are expressed by P19 embryonal cells. J Neurobiol 30:303-14
Johnson, D S; Heinemann, S F (1995) Embryonic expression of the 5-HT3 receptor subunit, 5-HT3R-A, in the rat: an in situ hybridization study. Mol Cell Neurosci 6:122-38
Lobron, C; Wevers, A; Damgen, K et al. (1995) Cellular distribution in the rat telencephalon of mRNAs encoding for the alpha 3 and alpha 4 subunits of the nicotinic acetylcholine receptor. Brain Res Mol Brain Res 30:70-6
Johnson, D S; Martinez, J; Elgoyhen, A B et al. (1995) alpha-Conotoxin ImI exhibits subtype-specific nicotinic acetylcholine receptor blockade: preferential inhibition of homomeric alpha 7 and alpha 9 receptors. Mol Pharmacol 48:194-9
Johnson, D S; Heinemann, S F (1995) Detection of 5-HT3R-A, a 5-HT3 receptor subunit, in submucosal and myenteric ganglia of rat small intestine using in situ hybridization. Neurosci Lett 184:67-70
Egebjerg, J; Kukekov, V; Heinemann, S F (1994) Intron sequence directs RNA editing of the glutamate receptor subunit GluR2 coding sequence. Proc Natl Acad Sci U S A 91:10270-4
Brose, N; Huntley, G W; Stern-Bach, Y et al. (1994) Differential assembly of coexpressed glutamate receptor subunits in neurons of rat cerebral cortex. J Biol Chem 269:16780-4
Chini, B; Raimond, E; Elgoyhen, A B et al. (1994) Molecular cloning and chromosomal localization of the human alpha 7-nicotinic receptor subunit gene (CHRNA7). Genomics 19:379-81
Hollmann, M; Maron, C; Heinemann, S (1994) N-glycosylation site tagging suggests a three transmembrane domain topology for the glutamate receptor GluR1. Neuron 13:1331-43

Showing the most recent 10 out of 46 publications