Abstract

Our objective in the proposed research is to develop optical probes (fluorescent dyes and chromophores) that can be used to (1) measure membrane potentials in nerve tissue and cells that are too small for microelectrode studies, (2) measure intracellular pH, (3) measure cellular free calcium, and (4) identify transformed cells and quantitate the antigenic makeup of cell surfaces. We also plan to determine the molecular mechanism by which these new probes carry out their functions. We intend to accomplish our objectives by careful design, synthesis characterization and evaluation of the new optical probes. Evaluation will be carried out with biological and model systems. Much effort will be directed toward development of the highly fluorescent and absorptive polymethine dyes as optical probes of biological systems.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS019353-04
Application #
3399404
Study Section
Biophysics and Biophysical Chemistry A Study Section (BBCA)
Project Start
1982-07-01
Project End
1987-03-31
Budget Start
1985-04-01
Budget End
1986-03-31
Support Year
4
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Carnegie-Mellon University
Department
Type
Schools of Arts and Sciences
DUNS #
052184116
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213

  Publications

Mujumdar, S R; Mujumdar, R B; Grant, C M et al. (1996) Cyanine-labeling reagents: sulfobenzindocyanine succinimidyl esters. Bioconjug Chem 7:356-62
Mujumdar, R B; Ernst, L A; Mujumdar, S R et al. (1993) Cyanine dye labeling reagents: sulfoindocyanine succinimidyl esters. Bioconjug Chem 4:105-11
Waggoner, A S; Ernst, L A; Chen, C H et al. (1993) PE-CY5. A new fluorescent antibody label for three-color flow cytometry with a single laser. Ann N Y Acad Sci 677:185-93
Yu, H; Ernst, L; Wagner, M et al. (1992) Sensitive detection of RNAs in single cells by flow cytometry. Nucleic Acids Res 20:83-8
Southwick, P L; Ernst, L A; Tauriello, E W et al. (1990) Cyanine dye labeling reagents--carboxymethylindocyanine succinimidyl esters. Cytometry 11:418-30
Mujumdar, R B; Ernst, L A; Mujumdar, S R et al. (1989) Cyanine dye labeling reagents containing isothiocyanate groups. Cytometry 10:11-9
Ernst, L A; Gupta, R K; Mujumdar, R B et al. (1989) Cyanine dye labeling reagents for sulfhydryl groups. Cytometry 10:3-10
Galbraith, W; Ryan, K W; Gliksman, N et al. (1989) Multiple spectral parameter imaging in quantitative fluorescence microscopy. I: Quantitation of bead standards. Comput Med Imaging Graph 13:47-60
George, E B; Nyirjesy, P; Pratap, P R et al. (1988) Impermeant potential-sensitive oxonol dyes: III. The dependence of the absorption signal on membrane potential. J Membr Biol 105:55-64
George, E B; Nyirjesy, P; Basson, M et al. (1988) Impermeant potential-sensitive oxonol dyes: I. Evidence for an ""on-off"" mechanism. J Membr Biol 103:245-53

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