The objective of this proposal is to determine the role of phosphorylation of the AChR in the localization and stabilization of the AChR at the synapse. Monoclonal antibodies to upsilon 1, the Mr43,phi-phi-phi protein kinase from Torpedo which we believe phosphorylates the AChR, will be produced and those cross-reacting with the mammalian neuromuscular junction will be further selected. These monoclonal antibodies will be used to isolate the mammalian upsilon 1 on an affinity column and to characterize its physical and biochemical properties. In addition, these monoclonal antibodies will be used to study the appearance of mammalian upsilon 1 during development in the rat neuromuscular junction and in muscle cells in culture. Finally, monoclonal antibodies to upsilon 1 will be microinjected into living muscle cells in culture to determine whether this protein kinase is involved in receptor clustering.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
2R01NS021623-02A1
Application #
3402915
Study Section
Neurological Sciences Subcommittee 1 (NLS)
Project Start
1984-04-01
Project End
1988-11-30
Budget Start
1985-12-01
Budget End
1986-11-30
Support Year
2
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Ernest Gallo Clinic and Research Center
Department
Type
DUNS #
173995366
City
Emeryville
State
CA
Country
United States
Zip Code
94608