Cerebral cavernous malformations (CCM), characterized by dilated sinusoidal vascular spaces lined by a single layer of endothelium, affect the central nervous system and may lead to neurological problems including bleeding and seizures. Mutations in the three genes, CCM1 (KRIT1, Krev1/Rap1a Interaction Trapped 1), CCM2 (OSM, malcavernin) and CCM3 (PDCD10, Programmed Cell Death Protein 10) have been shown to cause CCM. While recent in vitro studies have begun to illuminate the nature of CCM signaling, an in vivo, mechanistic understanding of CCM pathophysiology is lacking. Based on our recent data from in vitro cell culture studies, in vivo data from our mouse conditional knock-out of Ccm3 in neural cells, and intriguing observations in animal models from various groups, we hypothesize that CCM3 is a negative modulator of Akt signaling via interactions with PP2A, and CCM lesions are the consequence of altered expression of Akt and its downstream effectors. We further hypothesize that CCM3 signaling mediates an interaction between neural and endothelial cells and alteration of this interaction caused by neural CCM3 loss, results in a vascular phenotype. We propose to test these hypotheses using in vitro and in vivo approaches aimed at elaborating this novel Ccm3 signaling pathway and to investigate its potential influence on the interactions among various cell types within the neurovascular unit in CCM lesion development. We will perform studies aimed at clarifying the nature of Ccm3 interactions with the Akt signaling pathway. We will comprehensively study the mx1-Cre;Ccm3lox/lox mouse model that we have recently generated. Unlike other conditional Ccm3 knockouts, these mice survive long term and develop vascular pathology. The vascular pathology is characterized by dilated vessels throughout the brain and isolated lesions that are reminiscent of human CCM disease. We will take advantage of our findings to gain insight into CCM3 function and signaling. In addition, we propose to cross inducible VE-cadherin-CreER and GFAP-CreER to Ccm3lox/lox mice to study cell autonomous effects of CCM3 in endothelial cells and postnatal astrocytes. The proposed experiments are focused on identifying the molecular pathways relevant to CCM disease and on studying these pathways in vivo with a long-term goal of defining new and more effective therapies.

Public Health Relevance

Mutations in the gene Programmed Cell Death Protein 10 (PDCD10) cause Cerebral Cavernous Malformation 3 (CCM3), a vascular disorder mainly affecting the brain. The root causes of CCM are largely unknown. To study these causes, we propose to use a mouse model, Emx1-Cre;Ccm3lox/lox that we recently developed which leads, in part, to the same of abnormalities seen in humans suffering from CCM. These mice develop spontaneous vascular lesions identical to those in human CCM. By using a variety of assays, we plan to identify the molecular mechanisms that lead to the vascular pathology. We also propose to develop new models with inducible Cre lines that will allow us to modulate CCC3 expression in astrocytes or endothelial cells at postnatal stages to study cell specific effects of CCM3. A deeper understanding of the causes of CCM pathology at the cellular and molecular level in vivo will set the stage for efforts to define new and more effective therapies for this potentially debilitating and sometimes fatal disorder.

National Institute of Health (NIH)
National Institute of Neurological Disorders and Stroke (NINDS)
Research Project (R01)
Project #
Application #
Study Section
Brain Injury and Neurovascular Pathologies Study Section (BINP)
Program Officer
Koenig, James I
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Yale University
Schools of Medicine
New Haven
United States
Zip Code
Louvi, Angeliki; Nishimura, Sayoko; G√ľnel, Murat (2014) Ccm3, a gene associated with cerebral cavernous malformations, is required for neuronal migration. Development 141:1404-15
Louvi, Angeliki; Chen, Leiling; Two, Aimee M et al. (2011) Loss of cerebral cavernous malformation 3 (Ccm3) in neuroglia leads to CCM and vascular pathology. Proc Natl Acad Sci U S A 108:3737-42
Chen, Leiling; Tanriover, Gamze; Yano, Hiroko et al. (2009) Apoptotic functions of PDCD10/CCM3, the gene mutated in cerebral cavernous malformation 3. Stroke 40:1474-81
Tanriover, Gamze; Boylan, Arianne J; Diluna, Michael L et al. (2008) PDCD10, the gene mutated in cerebral cavernous malformation 3, is expressed in the neurovascular unit. Neurosurgery 62:930-8;discussion 938
Bilguvar, Kaya; Bydon, Mohamad; Bayrakli, Fatih et al. (2007) A novel syndrome of cerebral cavernous malformation and Greig cephalopolysyndactyly. Laboratory investigation. J Neurosurg 107:495-9
Seker, Askin; Pricola, Katie L; Guclu, Bulent et al. (2006) CCM2 expression parallels that of CCM1. Stroke 37:518-23
Guclu, Bulent; Ozturk, Ali K; Pricola, Katie L et al. (2005) Mutations in apoptosis-related gene, PDCD10, cause cerebral cavernous malformation 3. Neurosurgery 57:1008-13
Guclu, Bulent; Ozturk, Ali K; Pricola, Katie L et al. (2005) Cerebral venous malformations have distinct genetic origin from cerebral cavernous malformations. Stroke 36:2479-80