Electrical impedance myography (EIM) is a new, safe, non-invasive, and rapidly applied technique for muscle assessment that we have been developing in our laboratory for the past several years. In EIM, low- intensity alternating current of varying frequencies is passed through muscle tissue and the consequent surface voltage patterns assessed. The technique holds the promise of assisting with the diagnosis and assessment of many conditions that produce muscle atrophy and weakness, including muscular dystrophies, neurogenic illnesses (such as amyotrophic lateral sclerosis), inflammatory myopathies, disuse states, and muscle wasting of the elderly. Our current work in normal human subjects and those with a variety of neuromuscular disorders is confirming the potential value of this methodology. However, many questions remain regarding the nature of the measurements and their specific relationship with muscle pathology. In this proposal, we address these questions by performing detailed EIM measurements in rat models of neuromuscular disease and obtaining quantitative histomorphometry on the muscle tissue.
Our first aim will focus on further refining the technique for use in rats, confirming its reproducibility and identifying changes in EIM parameters with normal rat growth and aging.
The second aim will study a neurogenic disease model by following changes in muscle pathology and EIM measurements with degeneration and recovery from a nerve crush injury.
The third aim will evaluate the correlation between EIM measures in a model of inflammatory myositis.
The fourth aim will study muscle pathology and impedance change due to disuse using a hind limb suspension model. With these data, our current network models of underlying EIM theory will be further refined with the aim of being able to predict accurately muscle pathology from the EIM data. Additional outcomes of this work include: 1. learning how EIM parameters change with growth and aging;2. improving our ability to work with small muscles such that EIM can be used more effectively in children with muscular dystrophy and other disorders, and 3. to prepare the way for the use of EIM in screening of drugs and therapies in animals models of neuromuscular disease.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS055099-05
Application #
8050633
Study Section
Musculoskeletal Tissue Engineering Study Section (MTE)
Program Officer
Porter, John D
Project Start
2007-08-01
Project End
2012-04-30
Budget Start
2011-05-01
Budget End
2012-04-30
Support Year
5
Fiscal Year
2011
Total Cost
$327,995
Indirect Cost
Name
Beth Israel Deaconess Medical Center
Department
Type
DUNS #
071723621
City
Boston
State
MA
Country
United States
Zip Code
02215
Sanchez, Benjamin; Rutkove, Seward B (2017) Electrical Impedance Myography and Its Applications in Neuromuscular Disorders. Neurotherapeutics 14:107-118
Sanchez, Benjamin; Iyer, Shama R; Li, Jia et al. (2017) Non-invasive assessment of muscle injury in healthy and dystrophic animals with electrical impedance myography. Muscle Nerve 56:E85-E94
Hakim, Chady H; Mijailovic, Alex; Lessa, Thais B et al. (2017) Non-invasive evaluation of muscle disease in the canine model of Duchenne muscular dystrophy by electrical impedance myography. PLoS One 12:e0173557
Li, Jia; Yim, Sung; Pacheck, Adam et al. (2016) Electrical Impedance Myography to Detect the Effects of Electrical Muscle Stimulation in Wild Type and Mdx Mice. PLoS One 11:e0151415
Sanchez, Benjamin; Li, Jia; Geisbush, Tom et al. (2016) Impedance Alterations in Healthy and Diseased Mice During Electrically Induced Muscle Contraction. IEEE Trans Biomed Eng 63:1602-12
Arnold, W; McGovern, Vicki L; Sanchez, Benjamin et al. (2016) The neuromuscular impact of symptomatic SMN restoration in a mouse model of spinal muscular atrophy. Neurobiol Dis 87:116-23
Pacheck, Adam; Mijailovic, Alex; Yim, Sung et al. (2016) Tongue electrical impedance in amyotrophic lateral sclerosis modeled using the finite element method. Clin Neurophysiol 127:1886-90
Rutkove, Seward B; Wu, Jim S; Zaidman, Craig et al. (2016) Loss of electrical anisotropy is an unrecognized feature of dystrophic muscle that may serve as a convenient index of disease status. Clin Neurophysiol 127:3546-3551
Li, Jia; Pacheck, Adam; Sanchez, Benjamin et al. (2016) Single and modeled multifrequency electrical impedance myography parameters and their relationship to force production in the ALS SOD1G93A mouse. Amyotroph Lateral Scler Frontotemporal Degener 17:397-403
Wu, Jim S; Li, Jia; Greenman, Robert L et al. (2015) Assessment of aged mdx mice by electrical impedance myography and magnetic resonance imaging. Muscle Nerve 52:598-604

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