Prion protein (PrP) underlies a spectrum of diseases with no established treatment and devastating human and economic consequences. While substantial progress has been made in understanding the biochemical nature of the prion infectious agent, the lack of detailed structural information about the aggregated, disease-related forms of the prion protein (PrPSc) is a major barrier to progress in the field. In the current application, we propose to elucidate the structures of ordered, disease-related prion protein aggregates using state-of-the-art techniques including solid-state NMR methods supplemented with mass-per-length and volume-per-length measurements. The three specific aims are focused on elucidating the molecular structures of three homogeneous but conformationally distinct forms of the ordered PrP aggregates prepared in vitro using highly pure full-length recombinant PrP under well-defined conditions. They include PrPSc with a high infectivity titer and two types of PrP amyloid fibrils with limited infectivity or no detectible infectivity, respectively. When the goals of this project are accomplished, the current study will identify structural features that distinguish infectious and non- infectious states of self-propagating ordered PrP aggregates and define molecular determinants of prion infectivity. Ultimately, insight gained from this research will aid in the efforts to develop effective therapeutics for prion diseases and a better understanding of the mechanisms of prion replication. Furthermore, these studies will lay the ground work for understanding the mechanisms responsible for prion-like replication of abnormal conformation of non-prion proteins associated with other neurodegenerative diseases.

Public Health Relevance

Prion diseases are a group of fatal age-dependent neurodegenerative diseases that can arise spontaneously or be inherited, but can also be infectious. Lack of structural information about infectious, disease-related forms of the prion protein represents the major critical barrier to progress in the field. The current project seeks t elucidate the molecular structure of disease-related forms of the prion protein and define molecular determinants of prion infectivity.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS074998-04
Application #
8714080
Study Section
Biophysics of Neural Systems Study Section (BPNS)
Program Officer
Wong, May
Project Start
2011-08-01
Project End
2016-07-31
Budget Start
2014-08-01
Budget End
2015-07-31
Support Year
4
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Maryland Baltimore
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
City
Baltimore
State
MD
Country
United States
Zip Code
21201
Makarava, Natallia; Savtchenko, Regina; Alexeeva, Irina et al. (2016) New Molecular Insight into Mechanism of Evolution of Mammalian Synthetic Prions. Am J Pathol 186:1006-14
Katorcha, Elizaveta; Srivastava, Saurabh; Klimova, Nina et al. (2016) Sialylation of Glycosylphosphatidylinositol (GPI) Anchors of Mammalian Prions Is Regulated in a Host-, Tissue-, and Cell-specific Manner. J Biol Chem 291:17009-19
Katorcha, Elizaveta; Daus, Martin L; Gonzalez-Montalban, Nuria et al. (2016) Reversible off and on switching of prion infectivity via removing and reinstalling prion sialylation. Sci Rep 6:33119
Srivastava, Saurabh; Makarava, Natallia; Katorcha, Elizaveta et al. (2015) Post-conversion sialylation of prions in lymphoid tissues. Proc Natl Acad Sci U S A 112:E6654-62
Katorcha, Elizaveta; Makarava, Natallia; Savtchenko, Regina et al. (2015) Sialylation of the prion protein glycans controls prion replication rate and glycoform ratio. Sci Rep 5:16912
Katorcha, Elizaveta; Klimova, Nina; Makarava, Natallia et al. (2015) Loss of Cellular Sialidases Does Not Affect the Sialylation Status of the Prion Protein but Increases the Amounts of Its Proteolytic Fragment C1. PLoS One 10:e0143218
Srivastava, Saurabh; Baskakov, Ilia V (2015) Contrasting Effects of Two Lipid Cofactors of Prion Replication on the Conformation of the Prion Protein. PLoS One 10:e0130283
Makarava, Natallia; Savtchenko, Regina; Baskakov, Ilia V (2015) Two alternative pathways for generating transmissible prion disease de novo. Acta Neuropathol Commun 3:69
Klimova, Nina; Makarava, Natallia; Baskakov, Ilia V (2015) The diversity and relationship of prion protein self-replicating states. Virus Res 207:113-9
Katorcha, Elizaveta; Makarava, Natallia; Savtchenko, Regina et al. (2014) Sialylation of prion protein controls the rate of prion amplification, the cross-species barrier, the ratio of PrPSc glycoform and prion infectivity. PLoS Pathog 10:e1004366

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