The broad, long-term objective of the project is to understand the importance of neuronal calcium (Ca2+) signaling in pathogenesis of Alzheimer's disease (AD). Presenilins are transmembrane proteins localized to endoplasmic reticulum (ER). Missense mutations in presenilins account for 40% of familial AD (FAD) cases. Many FAD mutations in presenilins have been also linked to abnormal endoplasmic reticulum (ER) calcium (Ca2+) signaling. The main aim of the current proposal is to understand the connection between mutations in presenilins, dysregulation of neuronal ER Ca2+ signaling and synaptic loss and dysfunction in AD. Specifically, we will focus on testing the novel hypothesis that defects in ER Ca2+ signaling may lead to destabilization of """"""""mushroom spines"""""""" widely considered to be physical units for memory storage by attacking these aims:. 1. To investigate the importance of postsynaptic store-operated calcium (SOC) entry pathway downregulation in loss of mature synaptic spines in AD. Our preliminary data suggest that the increase in neuronal ER Ca2+ levels leads to a compensatory downregulation of neuronal store-operated Ca2+ entry pathway (nSOC). We discovered that the downregulation of nSOC occurs due to reduced expression of STIM2 protein, a master regulator of nSOC. We propose that reduction in synaptic nSOC causes destabilization and eventual elimination of mushroom spines, leading to loss of memories in FAD and aging brains. This hypothesis will be tested in experiments with PS1-FAD mouse model and STIM2 conditional knockout mouse model. 2. To investigate the connection between dysregulation of neuronal activity and destabilization of LTP- induced mature synaptic spines in AD. Our preliminary data indicate that appropriate pattern of neuronal activity is critical for maintenance of mature """"""""mushroom spines"""""""". We further discovered that abnormal ER Ca2+ signaling causes disruption of this pattern in PS1-FAD neurons. We will perform a series of experiments aimed at dissecting the connection between ER Ca2+ homeostasis, neuronal activity pattern and stability of mushroom spines in AD neurons. We will evaluate a crucial role of intracellular Ca2+ stores and SK family of Ca2+-activated potassium channels in this process. 3. To analyze the cross-talk of amyloid and calcium pathways for AD pathogenesis. A 42 oligomers influence neuronal Ca2+ signaling and neuronal activity via variety of pathways. In this aim we will investigate if some of the Ca2+-related targets and pathways explored in SA1 and SA2 may also apply to models of amyloid synaptotoxicity. These experiments will be performed with in vitro model of A 42 synaptotoxicity and with recently generated APP-KI mouse model of AD.

Public Health Relevance

The proposed project will have direct and immediate relevance for public health. Alzheimer's disease (AD) is a major cause of dementia in the elderly and an enormous health problem. The experiments described in the grant are aimed at testing specific hypothesis regarding pathogenesis of AD and will provide information critical for eventual development of the cure.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
1R01NS080152-01A1
Application #
8632540
Study Section
Special Emphasis Panel (ZRG1-MDCN-N (03))
Program Officer
Corriveau, Roderick A
Project Start
2013-08-15
Project End
2018-05-31
Budget Start
2013-08-15
Budget End
2014-05-31
Support Year
1
Fiscal Year
2013
Total Cost
$459,113
Indirect Cost
$170,363
Name
University of Texas Sw Medical Center Dallas
Department
Physiology
Type
Schools of Medicine
DUNS #
800771545
City
Dallas
State
TX
Country
United States
Zip Code
75390
Popugaeva, Elena; Pchitskaya, Ekaterina; Bezprozvanny, Ilya (2016) Dysregulation of neuronal calcium homeostasis in Alzheimer's disease - A therapeutic opportunity? Biochem Biophys Res Commun :
Zhang, Hua; Sun, Suya; Wu, Lili et al. (2016) Store-Operated Calcium Channel Complex in Postsynaptic Spines: A New Therapeutic Target for Alzheimer's Disease Treatment. J Neurosci 36:11837-11850
Fisher, Abraham; Bezprozvanny, Ilya; Wu, Lili et al. (2016) AF710B, a Novel M1/σ1 Agonist with Therapeutic Efficacy in Animal Models of Alzheimer’s Disease. Neurodegener Dis 16:95-110
Zhang, Hua; Liu, Jie; Sun, Suya et al. (2015) Calcium signaling, excitability, and synaptic plasticity defects in a mouse model of Alzheimer's disease. J Alzheimers Dis 45:561-80
Popugaeva, Elena; Pchitskaya, Ekaterina; Speshilova, Anastasiya et al. (2015) STIM2 protects hippocampal mushroom spines from amyloid synaptotoxicity. Mol Neurodegener 10:37
Popugaeva, Elena; Vlasova, Olga L; Bezprozvanny, Ilya (2015) Restoring calcium homeostasis to treat Alzheimer's disease: a future perspective. Neurodegener Dis Manag 5:395-8
Zhang, Hua; Wu, Lili; Pchitskaya, Ekaterina et al. (2015) Neuronal Store-Operated Calcium Entry and Mushroom Spine Loss in Amyloid Precursor Protein Knock-In Mouse Model of Alzheimer's Disease. J Neurosci 35:13275-86
Egorova, Polina; Popugaeva, Elena; Bezprozvanny, Ilya (2015) Disturbed calcium signaling in spinocerebellar ataxias and Alzheimer's disease. Semin Cell Dev Biol 40:127-33
Poguzhelskaya, Ekaterina; Artamonov, Dmitry; Bolshakova, Anastasia et al. (2014) Simplified method to perform CLARITY imaging. Mol Neurodegener 9:19
Popugaeva, Elena; Bezprozvanny, Ilya (2014) Can the calcium hypothesis explain synaptic loss in Alzheimer's disease? Neurodegener Dis 13:139-41

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