Unlike mice and humans, some vertebrate species generate a diverse primary antibody repertoire in gut- associated lymphoid tissues (GALT), where B cells mutate an initial repertoire limited in diversity by preferential use of a small number of V, (D) and J gene segments during immunoglobulin (Ig) gene rearrangement in the bone marrow. Antibody diversification in GALT occurs in an antigen (Ag)- and T cell- independent manner and, in some species (e.g. rabbits), is dependent on select members of the intestinal microbiota. Although humans and mice utilize a different strategy, generating a highly diverse primary antibody repertoire through combinatorial rearrangement of many different V, (D) and J gene segments, we hypothesize that the former strategy is conserved in some human and mouse B cell populations. Casola et al. (2004), for example, found that B cells lacking B cell receptors spontaneously developed germinal centers (GCs) in Peyer's patches. This observation, demonstrating that B cells in mouse GALT can be activated by signals derived from the intestinal microbiota in an Ag-independent manner, is strikingly reminiscent of the Ag-independent B cell activation by intestinal commensals that drives antibody repertoire diversification in rabbit GALT. Similarly, Weller et al. (2004) identified a circulating human B cel population that expresses a somatically hypermutated antibody repertoire, even in patients lacking functional CD40 or CD40L, and hence unable to mount T-dependent immune responses or support affinity maturation. These, among other, lines of evidence lead us to hypothesize that Ag- and T cell-independent antibody repertoire diversification in GALT is conserved in some primate and rodent B cell populations.

Public Health Relevance

This study will identify B cells in mouse gut-associated lymphoid tissues that diversify their antibody genes in an antigen-independent manner. The experiments will rely on gene-deficient mouse strains in which B cells either cannot be activated by T cell-dependent responses, or cannot respond to microbial components through innate receptors. Activated T-independent B cells will isolated from gut-associated lymphoid tissue and their V-D-J genes will be analyzed for evidence of somatic hypermutation, Ag-independent diversification and V gene usage.

Agency
National Institute of Health (NIH)
Type
Small Research Grants (R03)
Project #
1R03AI111170-01
Application #
8684964
Study Section
Cellular and Molecular Immunology - B Study Section (CMIB)
Program Officer
Rothermel, Annette L
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Loyola University Chicago
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
City
Maywood
State
IL
Country
United States
Zip Code
60153