Odontalgia, or toothache, is one of the most common types of pain experienced by both adults and children. The pain can be severe, leading to disruption of daily activities, including missed work, sleep loss, problems eating, and mood alterations. One of the primary mechanisms contributing to odontalgia is inflammation of the dental pulp, or pulpitis. Cold hypersensitivity is one of the hallmarks of pulpitis. Importantly, te molecular mechanisms involved in cold detection in teeth are unknown. Understanding the neurobiology of cold nociception by dental pulp afferents is important for both identifying new strategies for dental pain management and providing insight into the neurobiology of cold nociception. Multiple receptors are potentially involved in the detection and transduction of noxious and non-noxious cold, two of which belong to the transient receptor potential family, the TRPM8 and TRPA1 receptors. This proposal seeks to characterize pulpal afferents, in respect to TRPM8 and TRPA1 expression and function, in order to explicate their role in transducing noxious cold in teeth. Studies in Specific Aim 1A will evaluate TRPM8 and TRPA1 expression and function in the population of trigeminal sensory neurons innervating dental pulp using neuroanatomical methods.
Specific Aim 1 B will utilize pharmacologic and genetic approaches to evaluate TRPM8 and TRPA1 receptor activity in cells projecting to dental pulp using calcium microfluorometry.
Specific Aim 2 will evaluate the contribution of TRPM8 and TRPA1 to activation of central trigeminal nociceptive neurons upon noxious cold stimulation of dental pulp. As TRP receptors have emerged as critically important receptors for nociceptive signaling and integration, understanding their expression and function in pulpal fibers is key to understanding one of the most common types of orofacial pain, odontalgia. Further, these studies will contribute to our understanding of the neurobiology of receptors involved in cold nociception.

Public Health Relevance

Dental pain can be severe, causes disruption of activities of daily living, and disproportionately affects Americans with lower income. Despite the clear association of dental pain and cold hypersensitivity, the mechanisms of cold detection in the dental pulp are not clear. We will evaluate the mechanisms of cold pain in dental pulp in order to identify new ways to treat dental pain.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Small Research Grants (R03)
Project #
1R03DE023153-01A1
Application #
8584071
Study Section
Special Emphasis Panel (ZDE1-RK (12))
Program Officer
Kusiak, John W
Project Start
2013-06-21
Project End
2015-05-31
Budget Start
2013-06-21
Budget End
2014-05-31
Support Year
1
Fiscal Year
2013
Total Cost
$108,110
Indirect Cost
$33,110
Name
New York University
Department
None
Type
Schools of Dentistry
DUNS #
041968306
City
New York
State
NY
Country
United States
Zip Code
10012