Relapse to drug and food seeking presents a significant public health concern as excessive preoccupation with drug or food seeking and taking contributes to numerous negative health outcomes. Using a rat model of reward seeking, this application aims to identify gene expression patterns in the brains of rats with a history of sucrose self-administration under short or long-term abstinence conditions known to produce low or high levels, respectively, of reward seeking (""""""""incubation of craving""""""""). In addition, some rats will experience abstinence in an enriched environment, a manipulation we have previously demonstrated to attenuate incubation of sucrose seeking. Gene expression will be visualized in the first Aim using Fos immunohistochemistry to map sucrose-cue activated regions.
Aim 2 will utilize this mapping information to focus on discrete brain regions to isolate and quantitate dopamine D1 receptor signal cascade proteins, some in basal vs. phosphorylated states, as we have found that D1 receptor antagonism has time-dependent (incubation-dependent) effects on sucrose seeking. Beyond the goal of identifying neural substrates of addiction as a means to informing novel addiction therapies, the studies will be conducted as a means to expose undergraduate researchers to the scientific process. Engaging the students in this way will enhance their training experiences and the research environment at Western Washington University.

Public Health Relevance

Relapse to drug or food seeking presents a significant public health concern as excessive preoccupation with, and consumption of, drugs and food contribute to numerous negative health outcomes. The proposed studies aim to identify differential gene expression in the brains of rats related to relapse behavior with or without the relapse-attenuating pre-treatment of extended enriched environment living conditions. The results of these studies may lead to a better understanding of the molecular biology of relapse behavior and thus facilitate development of novel relapse treatment approaches.

National Institute of Health (NIH)
National Institute on Drug Abuse (NIDA)
Academic Research Enhancement Awards (AREA) (R15)
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Biobehavioral Regulation, Learning and Ethology Study Section (BRLE)
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Lynch, Minda
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Western Washington University
Schools of Arts and Sciences
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Glueck, Edwin; Ginder, Darren; Hyde, Jeff et al. (2017) Effects of dopamine D1 and D2 receptor agonists on environmental enrichment attenuated sucrose cue reactivity in rats. Psychopharmacology (Berl) 234:815-825
Slaker, Megan; Barnes, Jesse; Sorg, Barbara A et al. (2016) Impact of Environmental Enrichment on Perineuronal Nets in the Prefrontal Cortex following Early and Late Abstinence from Sucrose Self-Administration in Rats. PLoS One 11:e0168256
Harkness, John H; Wells, Jason; Webb, Sierra et al. (2016) Extended exposure to environmental cues, but not to sucrose, reduces sucrose cue reactivity in rats. Learn Behav 44:59-66
Grimm, Jeffrey W; Barnes, Jesse L; Koerber, Jonathon et al. (2016) Effects of acute or chronic environmental enrichment on regional Fos protein expression following sucrose cue-reactivity testing in rats. Brain Struct Funct 221:2817-30
Aoyama, Kenjiro; Barnes, Jesse; Koerber, Jon et al. (2016) Systemic injection of the DAD1 antagonist SCH 23390 reduces saccharin seeking in rats. Appetite 105:8-13
Aoyama, K; Barnes, J; Grimm, J W (2014) Incubation of saccharin craving and within-session changes in responding for a cue previously associated with saccharin. Appetite 72:114-22
Grimm, Jeffrey W; Weber, Rachel; Barnes, Jesse et al. (2013) Brief exposure to novel or enriched environments reduces sucrose cue-reactivity and consumption in rats after 1 or 30 days of forced abstinence from self-administration. PLoS One 8:e54164
Koerber, Jonathon; Goodman, David; Barnes, Jesse L et al. (2013) The dopamine D2 antagonist eticlopride accelerates extinction and delays reacquisition of food self-administration in rats. Behav Pharmacol 24:633-9
Grimm, Jeffrey W; Ratliff, Christine; North, Kindsey et al. (2012) Nicotine increases sucrose self-administration and seeking in rats. Addict Biol 17:623-33
Grimm, Jeffrey W; Harkness, John H; Ratliff, Christine et al. (2011) Effects of systemic or nucleus accumbens-directed dopamine D1 receptor antagonism on sucrose seeking in rats. Psychopharmacology (Berl) 216:219-33

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