The research progress of our lab was seriously affected by the hurricane Sandy that devastated the northeastern United States, especially New York City. On October 29th 2012, lower Manhattan, more specifically the area to the south of 40th Street suffered from an unexpected major power outage that lasted for a week. During this period, the power to the laboratory was completely shut down. All the fridges and freezers in the laboratory remained off during the power outage due to the lack of a backup generator in the building. A -80C freezers compressor was damaged due to the sudden power outage and the freezer had to be sent out for repair afterwards. The major power outage caused by hurricane Sandy caused severe losses to our laboratory materials. Lysates and cells, including costly cell line stocks, were stored in the -80C freezer. These valuable materials thawed and melted and were therefore damaged. Some of the lysate samples are very difficult to produce and collect. These samples required large amounts of time, work and expense to manufacture. They were generated from a large number of experimental animals including adult and pubertal mice through several rounds of a 10 hour STA-PUT separation procedure and were stored in the freezer for following immunoprecipitations (IP) and mass-spectrometry identifications. Numerous antibodies were required to confirm those IP experiments, all of which were stored in either a -20C freezer or 4C fridge. Consequently these antibodies were also spoiled during the power outage. Additionally, all reagents useful for the SUMO project (such as lysis buffer, media, western blot reagents etc.) were stored in the fridges and freezers and were also ruined. Due to the aforementioned losses caused by Sandy, more funds will be needed to replace the reagents, antibodies and personnel time.

Public Health Relevance

In at least half of all cases of human infertility (one in every six couples who are trying to conceive) male spermatogenic failure is a major or contributing cause. This work focuses on studies of the biological functions of novel proteins (SUMO) at the level of individual targets and corresponding pathways, leading to the better understanding of possible causes of male infertility and development of novel safe contraceptives, thus improving human healthcare. The research progress of the lab was seriously affected by the hurricane Sandy that devastated the northeastern United States, especially New York City. On October 29th 2012. During this period, the power to the laboratory was completely shut down. All the fridges and freezers in the laboratory remained off during the power outage due to the lack of a backup generator in the building. Due to the aforementioned losses caused by Sandy, more funds will be needed to replace the reagents, antibodies and personnel time.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Academic Research Enhancement Awards (AREA) (R15)
Project #
3R15HD067944-01A1S1
Application #
8742732
Study Section
Program Officer
Moss, Stuart B
Project Start
2013-12-06
Project End
2015-11-30
Budget Start
2013-12-06
Budget End
2015-11-30
Support Year
1
Fiscal Year
2014
Total Cost
$103,540
Indirect Cost
$41,540
Name
Albert Einstein College of Medicine
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
071036636
City
New York
State
NY
Country
United States
Zip Code
10033
Vigodner, Margarita; Shrivastava, Vibha; Gutstein, Leah Elisheva et al. (2013) Localization and identification of sumoylated proteins in human sperm: excessive sumoylation is a marker of defective spermatozoa. Hum Reprod 28:210-23