We propose a systematic proteomic analysis of the human innate immune response to DNA infection coupled with a functional analysis of genes in the network. We focus on the signaling pathways involved in transcription of type I interferon. This project provides a glimpse into the global architecture of antiviral signaling and serves as a resource for further mechanistic analysis of the pathway.
: The molecular mechanisms involved in regulating innate immune responses to DNA viruses are incompletely understood. We propose a systematic proteomic approach to discover the molecular signaling pathways controlling responses to DNA and DNA viruses.
|Fu, Bishi; Wang, Lingyan; Ding, Hao et al. (2015) TRIM32 Senses and Restricts Influenza A Virus by Ubiquitination of PB1 Polymerase. PLoS Pathog 11:e1004960|
|Li, Shitao; Wang, Lingyan; Fu, Bishi et al. (2014) Trim65: a cofactor for regulation of the microRNA pathway. RNA Biol 11:1113-21|
|Fu, Bishi; Li, Shitao; Wang, Lingyan et al. (2014) The ubiquitin conjugating enzyme UBE2L3 regulates TNF*-induced linear ubiquitination. Cell Res 24:376-9|
|Li, Shitao; Wang, Lingyan; Fu, Bishi et al. (2014) TRIM65 regulates microRNA activity by ubiquitination of TNRC6. Proc Natl Acad Sci U S A 111:6970-5|
|Wang, Lingyan; Li, Shitao; Dorf, Martin E (2012) NEMO binds ubiquitinated TANK-binding kinase 1 (TBK1) to regulate innate immune responses to RNA viruses. PLoS One 7:e43756|