Thrombospondin type-1 repeat (TSR) domains play essential roles in gliding motility, host-cell recognition and invasion throughout the life cycle o the malaria parasite, Plasmodium falciparum. These domains are present in proteins that are particularly important during parasite transmission from humans to mosquitoes and back.
The aim of this project is to explore and characterize the O- fucosylation of TSR domains of critical P. falciparum molecules. As it has been described across diverse organisms, TSR domains are commonly fucosylated by the protein-O-fucosyltransferase 2 (PoFUT2) and this modification is required for optimal folding and secretion of TSR-containing proteins. Furthermore, the O-fucosylation consensus sequence on TSR domains coincides with its ligand-binding motif, suggesting that O-fucose may alter ligand-binding affinities of TSR-domains. A PoFUT2 homolog is conserved and expressed by P. falciparum, and GDP-fucose, the substrate donor of O-fucosylation reactions, is actively synthesized and incorporated by the parasite. Together with the detection of the O-fucosylation machinery in salivary gland sporozoites by proteomic analyses, the evidence strongly point to the conservation of a PoFUT2 mediated O-fucosylation mechanism in P. falciparum. We propose to (1) explore these putative posttranslational modifications by characterizing two endogenously expressed and essential TSR-containing proteins in the ookinete and sporozoite stages (the Circumsporozoite and TRAP-related protein, CTRP; and the Circumsporozoite protein, CS, respectively) and (2) evaluate the biological significance of TSR modification by phenotyping O- fucosylation null mutants in the ookinete and sporozoite stages. TSR domains are essential for host- parasite interactions in malaria. A deeper insight into a mechanism of posttranslational modification of P. falciparum TSR will ultimately lay the foundation for future exploration into the fundamental role of glycosylation in malaria parasite biology.

Public Health Relevance

The thrombospondin type I repeat (TSR) domains of several key proteins of the malaria parasite facilitate attachment to human and mosquito host cells. We hypothesize that the Plasmodium biosynthetic machinery modifies these domains with the sugar fucose, thus our aim is to describe this modification and evaluate its biological significance during the parasite transmission from humans to mosquitoes and back. The discovery of new interventions to combat malaria remains greatly limited by our incomplete knowledge of basic Plasmodium falciparum biology, and the data generated by this proposal may ultimately contribute to the development of novel interventions to prevent parasite transmission.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Exploratory/Developmental Grants (R21)
Project #
1R21AI115063-01
Application #
8805027
Study Section
Membrane Biology and Protein Processing (MBPP)
Program Officer
Mcgugan, Glen C
Project Start
2014-12-15
Project End
2016-11-30
Budget Start
2014-12-15
Budget End
2015-11-30
Support Year
1
Fiscal Year
2015
Total Cost
$250,130
Indirect Cost
$67,890
Name
Johns Hopkins University
Department
Microbiology/Immun/Virology
Type
Schools of Public Health
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21205
Cova, Marta; Rodrigues, João A; Smith, Terry K et al. (2015) Sugar activation and glycosylation in Plasmodium. Malar J 14:427