Leucine rich repeat kinase 1 (Lrrk1) belongs to ROCO family proteins and contains three ankyrin repeat, seven leucine-rich repeat, a GTPase-like domain of Roc (Ras of complex proteins), COR (C-terminal of Roc) domain, and a serine/threonine kinase domain that is regulated by GTP binding to the Roc domain. We recently demonstrated that Lrrk1 knockout (KO) mice as well as a patient with a Lrrk1 mutation are severely osteopetrotic resulting from mature OC dysfunction and reduced bone resorption in the metaphyseal trabecular regions of the tubular and axial bones. These observations make Lrrk1 an ideal drug target for treatment of high bone turnover. Our focus in this grant is to develop small molecular weight inhibitors of Lrrk1 for treatment and prevention of osteoporosis and osteoporotic fractures. To this end, we propose two hypotheses in this study: 1) Lrrk1 inhibitor, IN04, inhibits elevated bone resorption (BR) but not bone formation (BF) in OVX mice; 2) INO4 inhibits Lrrk1 kinase activity by directly binding to the active site of the Lrrk1 kinase domain. To test the hypothesis 1, experiments are designed to compare the systemic effects of IN04 versus bisphosphonates on bone mass and strength in OVX- and Sham mice, by microCT, histomorphometric analyses, and mechanical property tests, and measure BR, BF markers in serum from vehicle treated or IN04 treated OVX mice at various time points. OC-secreted coupling factors in IN04-, bisphosphonates- or vehicle-treated OCs will also be examined by real-time PCR and Western blot analyses. To test the hypothesis 2, experiments are designed to express and purify recombinant human Lrrk1 kinase domain produced in E. coli cells, co-crystalize this kinase domain with ATP and IN04, and resolve the crystal structures. More potential Lrrk1 inhibitors will be screened by re-docking small molecules into the active pocket of the resolved Lrrk1 kinase domain. The results of this application will develop potential small molecular weight inhibitors of Lrrk1 for treatment and prevention of osteoporosis.

Public Health Relevance

Developing alternative anti-resorption drugs to treat osteoporosis, a major public health threat, would require structure-based drug design and high throughput screening of small molecular weight inhibitors from the chemical libraries, and pharmaceutical tests in vitro and in vivo. Successful completion of the proposed studies using structure-based drug screening and in vivo drug testing in estrogen-deficient mice should lead to development of novel inhibitors of leucine rich repeat kinase 1 (Lrrk1) for treatment of bone loss and weakness. Our confirmation of the effectiveness for human Lrrk1 inhibitor on bone resorption in mice will lead to further drug testing in large animals and humans. Identification of more specific Lrrk1 inhibitors will lead to novel therapeutic drugs for treatment of patients with bone diseases.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Exploratory/Developmental Grants (R21)
Project #
1R21AR072831-01
Application #
9434449
Study Section
Skeletal Biology Development and Disease Study Section (SBDD)
Program Officer
Alekel, D Lee
Project Start
2018-04-01
Project End
2020-01-31
Budget Start
2018-04-01
Budget End
2019-01-31
Support Year
1
Fiscal Year
2018
Total Cost
Indirect Cost
Name
Loma Linda Veterans Assn Research & Education
Department
Type
DUNS #
606630762
City
Redlands
State
CA
Country
United States
Zip Code
92373