This exploratory project is aimed at investigating the potential of newly developed optical interferometric technique that we developed- Spatial Light Interference Microscopy (SLIM)-- for characterizing blood smears and tissue biopsies. SLIM is label-free and uniquely sensitive to refractive index modifications brought to the biospecimen by disease development . If successful, in the long-run, i.e. 5-10 years period, we anticipate that this label-free approach to imaging biospecimens will provide faster access to clinically-relevant data, as it bypasses most of the histology preparation process, e.g. staining. Further, due to its exquisite sensitivity to structural changes in the tissue, SLIM will provide a greater wealth of information than common histology, which should aid the pathologist in reaching a faster, more accurate diagnosis. The exploratory stage project will have the following specific aims: develop advanced, high-throughput instrumentation, perform proof-of principle studies on blood smears with focus on several specific diseases, and image biopsies of normal and three types of cancers (breast, prostate, and lung).

Public Health Relevance

This exploratory project is aimed at investigating the potential of newly developed optical interferometric technique that we developed- Spatial Light Interference Microscopy (SLIM)-- for characterizing blood smears and tissue biopsies. SLIM is label-free and uniquely sensitive to refractive index modifications brought to the biospecimen by disease.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Exploratory/Developmental Grants (R21)
Project #
1R21CA147967-01
Application #
7852748
Study Section
Special Emphasis Panel (ZCA1-SRLB-V (J1))
Program Officer
Rasooly, Avraham
Project Start
2010-04-08
Project End
2012-03-31
Budget Start
2010-04-08
Budget End
2011-03-31
Support Year
1
Fiscal Year
2010
Total Cost
$175,198
Indirect Cost
Name
University of Illinois Urbana-Champaign
Department
Type
Organized Research Units
DUNS #
041544081
City
Champaign
State
IL
Country
United States
Zip Code
61820
Sridharan, Shamira; Katz, Aron; Soto-Adames, Felipe et al. (2015) Quantitative phase imaging of arthropods. J Biomed Opt 20:111212
Pham, Hoa V; Edwards, Christopher; Goddard, Lynford L et al. (2013) Fast phase reconstruction in white light diffraction phase microscopy. Appl Opt 52:A97-101
Pham, Hoa V; Bhaduri, Basanta; Tangella, Krishnarao et al. (2013) Real time blood testing using quantitative phase imaging. PLoS One 8:e55676
Kim, Taewoo; Sridharan, Shamira; Kajdacsy-Balla, Andre et al. (2013) Gradient field microscopy for label-free diagnosis of human biopsies [Invited]. Appl Opt 52:A92-6
Kim, Taewoo; Sridharan, Shamira; Popescu, Gabriel (2012) Gradient field microscopy of unstained specimens. Opt Express 20:6737-45
Pham, Hoa; Bhaduri, Basanta; Ding, Huafeng et al. (2012) Spectroscopic diffraction phase microscopy. Opt Lett 37:3438-40
Ben-Isaac, Eyal; Park, Yongkeun; Popescu, Gabriel et al. (2011) Effective temperature of red-blood-cell membrane fluctuations. Phys Rev Lett 106:238103
Ding, Huafeng; Wang, Zhuo; Liang, Xing et al. (2011) Measuring the scattering parameters of tissues from quantitative phase imaging of thin slices. Opt Lett 36:2281-3
Park, YongKeun; Best, Catherine A; Kuriabova, Tatiana et al. (2011) Measurement of the nonlinear elasticity of red blood cell membranes. Phys Rev E Stat Nonlin Soft Matter Phys 83:051925
Kim, Taewoo; Popescu, Gabriel (2011) Laplace field microscopy for label-free imaging of dynamic biological structures. Opt Lett 36:4704-6

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