This R21 proposal is submitted in response to Program Announcement Number PA-10-088 entitled "Exploratory Cancer Prevention Studies Involving Molecular Targets for Bioactive Food Components." Consumption of soy based foods has been shown to exhibit varying degrees of inhibitory effects on numerous types of cancers. Colon cancer is a common cause of cancer death in the United States. Diet and lifestyle factors, including obesity and lack of physical activity, are believed to be associated with increased colon cancer risk. Numerous observational studies in humans have indicated that a high consumption of isoflavones or soy in certain countries is associated with a reduced risk of colon cancer. However, the evidence for positive health effects of the isoflavones in soy is quite inconsistent. Rigorous mechanistic studies are needed to elucidate the cellular targets and mechanisms of action of soy compounds against colon and other cancers. We propose to combine analyses of protein structure and function with advanced methods of data management and drug screening to identify potential target proteins for three isoflavone soy compounds. We will use supercomputer technology combined with molecular biology, cell culture models and an in vivo mouse model to test and validate the computer prediction of the molecular target(s) of daidzein and three of its metabolites.

Public Health Relevance

Consumption of soy based foods has been shown to exhibit varying degrees of inhibitory effects on numerous types of cancers, including colon cancer, which is a very common cause of cancer death in the USA. Some studies suggest that consuming soy can reduce the risk of colon cancer whereas others suggest no effect or an increased risk. Rigorous mechanistic studies are needed to elucidate the cellular targets and mechanisms of action of soy compounds against colon cancer. We propose to combine analyses of protein structure and function with advanced methods of data management and drug screening to identify potential target proteins for three major isoflavones compounds found in soy.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Exploratory/Developmental Grants (R21)
Project #
5R21CA172457-02
Application #
8598082
Study Section
Chemo/Dietary Prevention Study Section (CDP)
Program Officer
Ross, Sharon A
Project Start
2013-01-01
Project End
2014-12-31
Budget Start
2014-01-01
Budget End
2014-12-31
Support Year
2
Fiscal Year
2014
Total Cost
$149,259
Indirect Cost
$51,384
Name
University of Minnesota Twin Cities
Department
None
Type
Organized Research Units
DUNS #
555917996
City
Minneapolis
State
MN
Country
United States
Zip Code
55455
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