The objective of the proposed application is to develop a dual functional probe which is detectable at single-particle level for both Raman and fluorescence microscopy, so that the strengths of each technique can be combined into a single imaging tool for tackling challenges in bioimaging. With this new imaging probe and tool, we plan to address a long-term bioimaging challenge that is real-time imaging of interactions between ligands and receptors at the molecular level inside live cells. Success of this proposed work will (1) provide a new imaging probe that is not only highly fluorescent and robust but can also report molecular information for ligands it labels;(2) offer a new imaging tool which integrates strengths of fluorescence microscopy and Raman spectroscopy;(3) enable us to correlate cellular dynamics of ligands with ligand-binding protein interactions at the chemical level inside live cells. The applications of these fluorescent and Raman probes are not limited to the proposed studies;they can also find applications in bioimaging as multiplexing and multifunctional probes to correlate fluorescence and Raman images with electron microscopy images at in vitro and in vivo level.
Ligand-receptor interactions play a pivotal role in regulating cellular functions and provide a foundation for disease diagnosis and new therapy development. However, comprehensive understanding of in vivo interactions between ligand and receptors at the molecular level remains highly challenging. Our research application aims to integrate fluorescence microscopy with Raman spectroscopy using a dual functional probe, so that we can simultaneously track and chemically image of ligand-receptor complexes at the single-cell level inside live cells. Success of this proposed work will (1) provide a new imaging probe that not only is highly fluorescent and robust but also can report molecular information of ligands it labels;(2) offer a new imaging tool which integrate strengths of fluorescence microscopy and Raman spectroscopy;(3) enable us to correlate cellular dynamics of ligands with ligand-receptor interactions at the chemical level inside live cells.
|Vinluan 3rd, Rodrigo D; Zheng, Jie (2015) Serum protein adsorption and excretion pathways of metal nanoparticles. Nanomedicine (Lond) 10:2781-94|
|Vinluan 3rd, Rodrigo D; Yu, Mengxiao; Gannaway, Melissa et al. (2015) Labeling Monomeric Insulin with Renal-Clearable Luminescent Gold Nanoparticles. Bioconjug Chem 26:2435-41|
|Zhao, Jianli; Sun, Shasha; Swartz, Logan et al. (2015) ""Size-Independent"" Single-Electron Tunneling. J Phys Chem Lett 6:4986-90|
|Sun, Shasha; Zhou, Chen; Chen, Sishan et al. (2014) Surface-chemistry effect on cellular response of luminescent plasmonic silver nanoparticles. Bioconjug Chem 25:453-9|
|Vinluan 3rd, Rodrigo D; Liu, Jinbin; Zhou, Chen et al. (2014) Glutathione-coated luminescent gold nanoparticles: a surface ligand for minimizing serum protein adsorption. ACS Appl Mater Interfaces 6:11829-33|
|Zhou, Chen; Yang, Shengyang; Liu, Jinbin et al. (2013) Luminescent gold nanoparticles: a new class of nanoprobes for biomedical imaging. Exp Biol Med (Maywood) 238:1199-209|
|Liu, Jinbin; Yu, Mengxiao; Ning, Xuhui et al. (2013) PEGylation and zwitterionization: pros and cons in the renal clearance and tumor targeting of near-IR-emitting gold nanoparticles. Angew Chem Int Ed Engl 52:12572-6|
|Liu, Jinbin; Yu, Mengxiao; Zhou, Chen et al. (2013) Passive tumor targeting of renal-clearable luminescent gold nanoparticles: long tumor retention and fast normal tissue clearance. J Am Chem Soc 135:4978-81|
|Yang, Shengyang; Zhou, Chen; Liu, Jinbin et al. (2012) One-step interfacial synthesis and assembly of ultrathin luminescent AuNPs/silica membranes. Adv Mater 24:3218-22|
|Zheng, Jie; Zhou, Chen; Yu, Mengxiao et al. (2012) Different sized luminescent gold nanoparticles. Nanoscale 4:4073-83|
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